Abstract
The enzymatic production of L-tryptophan from indole and L-serine, with pyridoxal phosphate as co-factor, can be achieved either with tryptophan — synthase or with tryptophanase. The low solubility of indole in the aqueous phase is a technological challenge common to both enzymatic routes. Attempts to circumvent this problem have included (i) the introduction of indole in a separate phase and (ii) fed-batch operation.
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References
Ribeiro, M.H.L., Cabrai, J.M.S. and Fonseca, M.M.R. (1987) ‘Production of L-tryptophan in a Two-liquid-phase System’, in Biocatalysis in Organic Media, C. Laane, J. Tramper and M.D. Lilly (eds.), Elsevier, Amsterdam, pp. 323–329.
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© 1992 Springer Science+Business Media Dordrecht
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Ribeiro, M.H.L., Nunes, I.M.L., Cabral, J.M.S., Fonseca, M.M.R. (1992). A Multiphase System for the Production of L-Tryptophan from L-Serine and Indole; Studies on Product Separation and Recovery. In: Vardar-Sukan, F., Sukan, Ş.S. (eds) Recent Advances in Biotechnology. NATO ASI Series, vol 210. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2468-3_46
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DOI: https://doi.org/10.1007/978-94-011-2468-3_46
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