Skip to main content
SpringerLink
Log in
Book cover

Recent Advances in Biotechnology pp 449–454Cite as

Membrane Bioreactors for Animal Cell Culture

  • Chapter

  • 640 Accesses

Part of the book series: NATO ASI Series ((NSSE,volume 210))

Abstract

Various types of membrane bioreactors (flat sheet, coiled tube, multitubular) were developed and used for monoclonal antibody (MAb) formation by mouse-mouse hybridoma cell lines. The effects of different molecular weight (MW) cutoff membranes on growth and antibody (IgG) formation were investigated. Nutrient and toxic product diffusion through the membranes were quantified and kinetic and physical constants of the system were determined using a suitable mathematical model

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Birch, J.R Thompson, P., Laumbert, K and Boraston, R. (1985). in J. Feder and W Tolbert (eds). Large Scale Mammalian cell culture, Academic Press, New York, pp 1–15.

    Google Scholar 

  2. Hirschel, M.D. and Gruenberg, M.L. (1987), in B. Lydersen (ed). Large scale cell culture Technology, Hanser Publishers, pp 115-144.

    Google Scholar 

  3. Altshuler, G., Dziewulski, D, Sowek, J and Belfort, G. (1982). Biotechnol. Bioeng 31, 168–172.

    Google Scholar 

  4. Hagedorn, J. and Kargì, F. (1990) Production of Monoclonal Antibodies bv Hybridoma cells in a Flat Sheet Membrane Bioreactor. Biotechnol Progress. 6, 220–224.

    Article  CAS  Google Scholar 

  5. Hagedorn, J. and Kargì, F. (1990) Coiled Tube membrane bioreactor for cultivation of hybridoma cells producing monoclonal antibodies Enzyme Wicrob. Technol. 12, 824–829.

    CAS  Google Scholar 

  6. Sigma Glucose Diagnostic Kit, Procedure 115, Sigma Chemical Company, st. Lovis, MO.

    Google Scholar 

  7. Sigma pyruvate/lactate Diagnostic Kit Procedure 726 UV/826 Sigma Chemical Co. St. Louis. Mo.

    Google Scholar 

  8. Sigma Ammonia Diagnostic Kit. Procedure 170-UV. Sigma Chemical CO. St. Louis. MO.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Environmental Eng., Dokuz Eylül University, Bornova — Izmir, Turkey

    Dr. Fikret Kargi

Authors
  1. Dr. Fikret Kargi
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Chemical Engineering, Ege University, Izmir, Turkey

    Fazilet Vardar-Sukan

  2. Biotechnology Centre and Department of Food Engineering, Ege University, Izmir, Turkey

    Ş. Suha Sukan

Rights and permissions

Reprints and permissions

Copyright information

© 1992 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Kargi, F. (1992). Membrane Bioreactors for Animal Cell Culture. In: Vardar-Sukan, F., Sukan, Ş.S. (eds) Recent Advances in Biotechnology. NATO ASI Series, vol 210. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2468-3_30

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-2468-3_30

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5089-0

  • Online ISBN: 978-94-011-2468-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation