Abstract
This chapter describes the protocols necessary for the co-transfection of Sf cells with virus DNA and transfer vector, and the subsequent detection, separation and purification of recombinant viruses. Before attempting to make a recombinant virus you should ensure that you: (1) have purified AcMNPV or AcMNPY.lacZ DNA, as appropriate (see Chapter 6); (2) have checked that the foreign gene(s) is inserted into the appropriate transfer vector in the correct orientation (see Chapter 4); (3) can carry out a plaque-assay (see Chapter 6).
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© 1992 L. A. King and R. D. Possee
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King, L.A., Possee, R.D. (1992). Production and selection of recombinant virus. In: The Baculovirus Expression System. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2374-7_7
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DOI: https://doi.org/10.1007/978-94-011-2374-7_7
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5047-0
Online ISBN: 978-94-011-2374-7
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