Skip to main content
SpringerLink
Log in

Effect of Na+ electrochemical potential energy on system A Amino acid transport in rat skeletal muscle during endotoxic shock

  • Chapter
Amino Acids

  • 29 Accesses

Abstract

We previously reported a decrease in Na+ dependent amino acid (AA) transport by endotoxic rat soleus muscle, in vitro. This study evaluated the kinetics of AA transport in vitro, active AA transport in vivo and intra- and extracellular Na+ concentration (mmol/L) and the calculated chloride equilibrium potential (mv) in endotoxic soleus muscle. Fasted male rats (90 g) received an i.v. injection of the system A AA analog alpha-aminoisobutyric acid (AIB) (1 mg/kg) and Salmonella enteritidis endotoxin (ETX) (20 mg/kg) or saline (SAL) and were killed 4–5 h later. Kinetic parameters Vmax (nmol/g dry wt/min) and Km (mM) were estimated from initial rates of AIB uptake during incubations in O2-Krebs with various [AIB] (0.1–20 mM). AIB uptake by muscle in vivo was expressed as a distribution ratio (DR; [AIB]intracell/ [AIBJextracell) to assess active AA transport.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 74.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Karlstad MD and Sayeed MM (1985) Am. J. Physiol. (Regulatory, Integrative Comp. Physiol. 17) 248: R142 - R146.

    Google Scholar 

  2. Karlstad MD and Sayeed MM (1986) Am. J. Physiol. (Regulatory, Integrative Comp. Physiol. 20) 251: R150 - R156.

    Google Scholar 

  3. Karlstad MD and Sayeed MM (1987) Am. J. Physiol. (Regulatory, Integrative Comp. Physiol. 21) 252: R674 - R680.

    Google Scholar 

  4. Jepson MM, Pall JM, Bates PC and Millward DJ (1986) Biochem. J. 235: 329–336.

    PubMed  CAS  Google Scholar 

  5. Hasseigren PO, James JH and Fischer JE (1986) Ann. Surg. 203: 360–365.

    Article  Google Scholar 

  6. Moore RD (1983) Biochim. Biophys. Acta 737: 1–49.

    PubMed  CAS  Google Scholar 

  7. Narahara HT and Holloszy JO (1974) J. Biol. Chem. 249, 5435–5443.

    PubMed  CAS  Google Scholar 

  8. Parrish JE and Kipnis DM (1964) J. Clin. Invest. 43: 1994–2002.

    Article  PubMed  CAS  Google Scholar 

  9. Clowes GHA Jr., O’Donnell TF, Blackburn GL and Maki TN (1976) 56: 1169–1184.

    CAS  Google Scholar 

  10. Rosenblatt S, Clowes GHA Jr., George BC, Hirsch E and Lindberg B (1983) Arch. Surg. 118: 167–175.

    PubMed  CAS  Google Scholar 

  11. Williams JA, Withrow CD and Woodbury DM (1971) J. Physiol. (London) 212: 117–128.

    Google Scholar 

  12. Cotlove E (1961) Stand. Meth. Clin. Chem. 3: 81–98.

    CAS  Google Scholar 

  13. Kipnis DM and Parrish JE (1965) Fed. Proc. 24: 1051–1059.

    PubMed  CAS  Google Scholar 

  14. Lineweaver H and Burk D (1934) J. Am. Chem. Soc. 56: 658–666.

    Article  CAS  Google Scholar 

  15. Goldberg AL and Goodman HM (1969) Am. J. Physiol. 216: 1111–1115.

    PubMed  CAS  Google Scholar 

  16. Riggs TR and Walker LM (1963) Endocrinology 73: 781–788.

    Article  PubMed  CAS  Google Scholar 

  17. Akedo H and Christensen HN (1962) J. Biol. Chem. 237: 118–122.

    PubMed  CAS  Google Scholar 

  18. Riggs TR and McKirahan KJ (1973) J. Biol. Chem. 248: 6450–6455.

    PubMed  CAS  Google Scholar 

  19. Crane RK (1977) Rev. Physiol. Biochem. Pharmacol. 78: 99–159.

    Article  PubMed  CAS  Google Scholar 

  20. Geck P and Heinz E (1976) Biochim. Biophys. Acta 443: 49–53.

    Article  PubMed  CAS  Google Scholar 

  21. Sayeed MM (1984) Am. J. Physiol. (Regulatory, Integrative Comp. Physiol. 16) 247: R465 - R474.

    Google Scholar 

  22. Flear CTG (1970) J. Clin. Path. 23: 16–31.

    Article  Google Scholar 

  23. Flear CTG, Bhattacharya S and Singh CM (1980) JPEN 4: 98–120.

    Article  CAS  Google Scholar 

  24. Illner HP and Shires GT (1981) Arch. Surg. 116: 1302–1305.

    PubMed  CAS  Google Scholar 

  25. Gibson WH, Cook JJ, Gatipon G and Moses ME (1977) Surgery 81: 571–577.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Medical Biology, The University of Tennessee Medical Center at Knoxville, Knoxville, TN, 37920, USA

    Michael D. Karlstad

  2. Department of Anesthesiology, The University of Tennessee Medical Center at Knoxville, Knoxville, TN, 37920, USA

    John T. Chesney

  3. Department of Anesthesiology, University of Tennessee Medical Center, 1924 Alcoa Highway, Knoxville, TN, 37920, USA

    Michael D. Karlstad

Authors
  1. Michael D. Karlstad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John T. Chesney
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Pediatrics, University of Vienna, A-1090, Vienna, Austria

    Gert Lubec

  2. T. H. Morgan School of Biological Sciences and The Graduate Center for Toxicology, University of Kentucky, 40506-0225, Lexington, Kentucky, USA

    Gerald A. Rosenthal

Rights and permissions

Reprints and permissions

Copyright information

© 1990 ESCOM Science Publishers B.V.

About this chapter

Cite this chapter

Karlstad, M.D., Chesney, J.T. (1990). Effect of Na+ electrochemical potential energy on system A Amino acid transport in rat skeletal muscle during endotoxic shock. In: Lubec, G., Rosenthal, G.A. (eds) Amino Acids. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2262-7_9

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-2262-7_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-72199-04-1

  • Online ISBN: 978-94-011-2262-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation