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Occurrence and distribution of carotenoids in photosynthetic systems

  • Chapter
Carotenoids in Photosynthesis

Abstract

In the past few years the analysis of carotenoids has become much more sensitive with the introduction of high-performance liquid chromatography (HPLC), especially with diode-array detection. This has permitted much more accurate determination of the pigment content and composition of photosynthetic organisms. However, as a note of caution, whilst analytical techniques have improved greatly, they still require very careful extraction of the plant material and subsequent careful handling of the isolated carotenoids. Improper practices at this stage can lead to the formation of a number of artifacts (see Chapter 10). Particularly common is the production of the furanoid derivatives from carotenoids that contain 5,6-epoxide groups. Similarly, many workers continue to employ saponification as an obligatory step during the extraction procedure; at the very least this will cause minor losses of the carotenoids present but it may change the whole nature of the extract by removing xanthophyll esters or destroying some carotenoids.

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Young, A.J. (1993). Occurrence and distribution of carotenoids in photosynthetic systems. In: Young, A.J., Britton, G. (eds) Carotenoids in Photosynthesis. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2124-8_2

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