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Molecular Chaperones pp 79–85Cite as

The role of molecular chaperones in protein transport into the endoplasmic reticulum

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Summary

In eukaryotic cells export of the vast majority of newly synthesized secretory proteins is initiated at the level of the membrane of the endoplasmic reticulum (microsomal membrane). The precursors of secretory proteins are not transported across the microsomal membrane in their native state. Typically, signal peptides in the precursor proteins are involved in preserving the transport-competent state. Furthermore, there are two alternatively acting mechanisms involved in preserving transport competence in the cytosol. The first mechanism involves two ribonucleoparticles (ribosome and signal recognition particle) and their receptors on the microsomal surface and requires the hydrolysis of GTP. The second mechanism does not involve ribonucleoparticles and their receptors but depends on the hydrolysis of ATP and on cis-acting molecular chaperones, such as heat shock cognate protein 70 (hsc 70). In both mechanisms a translocase in the microsomal membrane mediates protein translocation. This translocase includes a signal peptide receptor on the cis-side of the microsomal membrane and a component that also depends on the hydrolysis of ATP. At least in certain cases, an additional nucleoside triphosphate-requiring step is involved which is related to the trans-acting molecular chaperone BiP.

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Authors
  1. T. Dierks
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  2. P. Klappa
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  3. H. Wiech
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  4. R. Zimmerman
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Editor information

Editors and Affiliations

  1. Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

    R. J. Ellis (Professor of Biological Sciences) (Professor of Biological Sciences)

  2. Wellcome/CRC Institute of Cancer and Developmental Biology, Cambridge University, Tennis Court Road, Cambridge, CB2 1QR, UK

    R. A. Laskey

  3. Central Research and Development Department, DuPont Company Experimental, Station E402, Wilmington, Delaware, 19880, USA

    G. H. Lorimer

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© 1993 Springer Science+Business Media Dordrecht

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Dierks, T., Klappa, P., Wiech, H., Zimmerman, R. (1993). The role of molecular chaperones in protein transport into the endoplasmic reticulum. In: Ellis, R.J., Laskey, R.A., Lorimer, G.H. (eds) Molecular Chaperones. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2108-8_10

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  • DOI: https://doi.org/10.1007/978-94-011-2108-8_10

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4935-1

  • Online ISBN: 978-94-011-2108-8

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