Skip to main content

Enzyme electrophoresis, genetic identity and description of a new genus and species of heteronemertean (Nemertea, Anopla) from northwestern Spain and North Wales

  • Conference paper
Advances in Nemertean Biology

Part of the book series: Developments in Hydrobiology ((DIHY,volume 89))

Abstract

The anoplan order Heteronemertea, particularly the genera Cerebratulus, Lineus and Micrura,contains a very large number of nominate species, many of which are inadequately described. As a consequence, systematic difficulties are encountered with the identification of many taxa in this group, especially those originally established primarily on the basis of their external features. The present paper concerns heteronemerteans collected from two locations, the Foz Estuary (north-western Spain) and Llandudno (North Wales). The Spanish collection included specimens identified as Lineus longissimus (Gunnerus), whilst samples from Llandudno contained large numbers of Lineus viridis (Müller); samples of a third similar but apparently undescribed species were found at both locations. Starch gel electrophoresis showed that samples of the apparent third species were genetically almost identical from each of the two locations, but were clearly different from the two described Lineus species. Histological studies of the unknown specimens revealed anatomical characters, including the unique feature of a proboscis epithelium ciliated throughout its length, which exclude it from any known heteronemertean taxon; it is accordingly placed in a new genus and species, for which the name Riseriellus occultus is proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ayala, F. J., 1975. Genetic differentiation during the speciation process. In T. Dobzhansky, M. K. Hecht & W. C. Steere (eds), Evolutionary biology. Plenum Press, New York 8: 1–78.

    Google Scholar 

  • Ayala, F. J., 1983. Enzymes as taxonomic characters. In G. S. Oxford & D. Rollinson (eds), Protein polymorphism: adaptive and taxonomic significance. Academic Press, London: 3–26

    Google Scholar 

  • Balakirev, E. S. & G. P. Manchenko, 1984. Vnutrividovaya geneticheskaya izmenchivost belkov u nemertinui Linens torquatus. Genetika 20: 2070–2071.

    Google Scholar 

  • Berg, G., 1972. Taxonomy of Amphiporus lactforeus (Johnston, 1828) and Amphiporus dissimulans Riches, 1893 (Nemertini, Hoplonemertini). Astarte 5: 19–26.

    Google Scholar 

  • Cantell, C.-E., 1972. Studies on the morphology, taxonomy, and larval development of heteronemertines (Nemertina). Acta Univ. Uppsala 218: 1–7.

    Google Scholar 

  • Cantell, C.-E.,1975. Anatomy, taxonomy, and biology of some Scandinavian heteronemertines of the genera Lineus, Micrura and Cerebratulus. Sarsia 58: 89–122.

    Google Scholar 

  • Cantell, C.-E., 1976. Complementary description of the morphology of Lineus longissimus(Gunnerus, 1770) with some remarks on the cutis layer in heteronemertines. Zool. Scr. 5: 117–120.

    Article  Google Scholar 

  • Cantell, C.-E. & L. Gidholm, 1977. Isozyme studies of two related nemertines, Micrura fasciolataEhrenberg 1831, and Micrura balticaCantell 1975. Zoon 5: 87–89.

    Google Scholar 

  • Fairburn, D. J. & D. A. Roth, 1980. Testing genetic models of isozyme variability without breeding data: can we depend on the X2 ? Can. J. Fish. aquat. Sci. 37: 1149–1159.

    Article  Google Scholar 

  • Ferguson, A., 1980. Biochemical systematics and evolution. Blackie & Son, Glasgow.

    Google Scholar 

  • Friedrich, H., 1935. Studien zur Morphologie, Systematik und Ökologie der Nemertinen der Kieler Bucht. Arch. Naturgesch. 4: 293–375.

    Google Scholar 

  • Friedrich, H., 1960. Bemerkungen Uber die Gattung MicruraEhrenberg 1831 und zur Klassifikation der Heteronemertinen nebst vorläufigem Bestimmungsschlüssel. Veröff. Inst. Meeresforsch. Bremerh. 7: 48–62.

    Google Scholar 

  • Gibson, R., 1982a. British nemerteans. University Press, Cambridge.

    Google Scholar 

  • Gibson, R., 1982b. Nemertea. In S. P. Parker (ed.), Synopsis and classification of living organisms. McGraw-Hill, New York 1: 823–846.

    Google Scholar 

  • Gibson, R., 1985. The need for a standard approach to taxonomic descriptions of nemerteans. Am. Zool. 25: 5–14.

    Google Scholar 

  • Gibson, R., 1990a. The macrobenthic nemertean fauna of Hong Kong. In B. Morton (ed.), Proceedings of the second international marine biological workshop: the marine flora and fauna of Hong Kong and southern China. University Press, Hong Kong 1: 33–212.

    Google Scholar 

  • Gibson, R., 1990b. The macrobenthic nemertean fauna of the Albany region, Western Australia. In F. E. Wells, D. I. Walker, H. Kirkman & R. Lethbridge (eds), Proceedings of the third international marine biological workshop: the marine flora and fauna of Albany, Western Australia. Western Australian Museum, Perth 1: 89–194.

    Google Scholar 

  • Gibson, R. & F. B. Crandall, 1989. The genus Amphiporus Ehrenberg (Nemertea, Enopla, Monostiliferoidea). Zool. Scr. 18: 453–470.

    Article  Google Scholar 

  • Gibson, R. & Qi Sang, 1991. A new freshwater heteronemertean from the Zhujiang (Pearl River), People’s Republic of China. Hydrobiologia 220: 167–178.

    Article  Google Scholar 

  • Gontcharoff, M. & H. Lechenault, 1958. Sur le déterminisme de la ponte chez Lineus lacteus. C. r. hebd. Séanc. Acad. Sci., Paris 246: 1630–1632.

    Google Scholar 

  • Harris, H. & D. A. Hopkinson, 1978. Handbook of enzyme electrophoresis in human genetics. North-Holland, Amsterdam.

    Google Scholar 

  • Hedrick, P. W., M. E. Ginevan & E. P. Ewing, 1976. Genetic polymorphism in heterogeneous environments. Annu. Rev. Ecol. Syst. 7: 1–32.

    Article  Google Scholar 

  • Lewontin, R. C., 1958. A general method for investigating the equilibrium of gene frequencies in a population. Genetics 43: 419–434.

    CAS  PubMed  Google Scholar 

  • Nei, M., 1972. Genetic distance between populations. Am. Nat. 106: 283–292.

    Article  Google Scholar 

  • Nevo, E., 1978. Genetic variation in natural populations: patterns and theory. Theoret. Pop. Biol. 13: 121–177.

    Article  CAS  Google Scholar 

  • Nevo, E., A. Beiles & R. Ben-Schlomo, 1984. The evolutionary significance of genetic diversity: ecological, demographic and life history correlates. In G. S. Mani (ed.), Evolutionary dynamics of genetic diversity. Springer-Verlag, Heidelberg: 13–212.

    Chapter  Google Scholar 

  • Norenburg, J. L., 1985. Structure of the nemertine integument with consideration of its ecological and phylogenetic significance. Am. Zool. 25: 37–51.

    Google Scholar 

  • Norenburg, J. L., 1993. Riserins pugetensis gen. n. sp. n. (Nemertina: Anopla), a new mesopsammic species, and comments on phylogenetics of some anoplan characters. Hydrobiologia 266: 203–218.

    Article  Google Scholar 

  • Pantin, C. F. A., 1960. Notes on microscopical technique for zoologists. University Press, Cambridge.

    Google Scholar 

  • Richardson, B. J., P. M. Baverstock & M. Adams, 1986. Allozyme electrophoresis Academic Press, Sydney.

    Google Scholar 

  • Riser, N. W., 1990. New Zealand nemertines from kelp hold-fasts: Heteronemertinea 1. Adenorhagas aurantiafrons gen. n., sp. n. N.Z. Jl Zool. 17: 597–606.

    Google Scholar 

  • Selander, R. K., 1977. Genetic variation in natural populations. In F. J. Ayala (ed.), Molecular evolution. Sinauer Associates, Sunderland, Mass.: 21–45.

    Google Scholar 

  • Solé-Cava, A. M. & J. P. Thorpe, 1990. High levels of genetic variation in marine sponges. In K. Riitzler (ed.), New perspectives in sponge biology. Smithsonian Institution Press, Washington, D.C.: 332–337.

    Google Scholar 

  • Solé-Cava, A. M. & J. P. Thorpe, 1991. High levels of genetic variation in natural populations of marine lower invertebrates. Biol. J. linn. Soc. 44: 65–80.

    Article  Google Scholar 

  • Stunkard, H. W., 1982. Mesozoa. In S. P. Parker (ed.), Synopsis and classification of living organisms. McGraw-Hill, New York 1: 853–855.

    Google Scholar 

  • Sundberg, P., 1979. Statistical analysis of variation in characters in Tetrastemma laminariae (Nemertini), with a redescription of the species. J. Zool., Lond. 189: 39–56.

    Article  Google Scholar 

  • Sundberg, P., 1980. Intraspecific variation in Tetrastemma laminariae (Nemertini): an examination of one of its possible causes. J. Zool., Lond. 192: 137–141.

    Article  Google Scholar 

  • Sundberg, P. & K. Janson, 1988. Polymorphism in Oerstedia dorsalis (Abildgaard, 1806) revisited. Electrophoretic evidence for a species complex. Hydrobiologia 156: 93–98.

    Article  Google Scholar 

  • Thorpe, J. P., 1982. The molecular clock hypothesis: biochemical evolution, genetic differentiation and systematics. Annu. Rev. Ecol. Syst. 13: 139–168.

    Article  CAS  Google Scholar 

  • Thorpe, J. P., 1983. Enzyme variation, genetic distance and evolutionary divergence in relation to levels of taxonomic separation. In G. S. Oxford & D. Rollinson (eds), Protein polymorphism: adaptive and taxonomic significance. Systematics Association, London: 131–152.

    Google Scholar 

  • Valenzuela, C. Y., 1985. Algebraic and epistemological restrictions in studies on Hardy-Weinburg equilibrium. Am. Nat. 125: 744–746.

    Article  Google Scholar 

  • Vernet, G., 1990. Rhopalura linei, Orthonectide parasite de l’Hétéronémerte Lineus ruber. Cah. Biol. mar. 31: 251–255.

    Google Scholar 

  • Walsh, P. J. & G. N. Somero, 1981. Temperature adaptation in sea anemones. Physiological and biochemical variability in geographically separate populations of Metridium senile. Mar. Biol. 62: 25–34.

    Article  CAS  Google Scholar 

  • Ward, R. D. & J. A. Beardmore, 1977. Protein variation in the plaice (Pleuronectes platessa). Genetic Res. 30: 45–62.

    Article  CAS  Google Scholar 

  • Wijnhoff, G., 1914. The proboscidian system in nemertines. Q. Jl microsc. Sci. 60: 273–312.

    Google Scholar 

  • Williams, K. A., D. O. F. Skibinski & R. Gibson, 1983. Isoenzyme differences between three closely related species of Lineus (Heteronemertea). J. exp. mar. Biol. Ecol. 66: 207–211.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

R. Gibson J. Moore P. Sundberg

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer Science+Business Media Dordrecht

About this paper

Cite this paper

Rogers, A.D., Junoy, J., Gibson, R., Thorpe, J.P. (1993). Enzyme electrophoresis, genetic identity and description of a new genus and species of heteronemertean (Nemertea, Anopla) from northwestern Spain and North Wales. In: Gibson, R., Moore, J., Sundberg, P. (eds) Advances in Nemertean Biology. Developments in Hydrobiology, vol 89. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2052-4_16

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-2052-4_16

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4909-2

  • Online ISBN: 978-94-011-2052-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics