Abstract
A small intestinal epithelial cell line, IEC-6, was analyzed for its growth and differentiation pattern in vitro. After IEC-6 cells had reached confluence, alkaline phosphatase (ALP), a differentiation marker, was introduced and its activity increased thereafter. Histochemical staining of the cells indicated that most of the ALP-positive cells did not proliferate. These results indicate that the cells differentiated during culture, which accompanied the end of proliferation. Furthermore, we found that the cells which became detached from the cultured plates had undergone apoptosis (programmed cell death). The differentiation of IEC-6 cells in the culture closely resembled the in vivo differentiation of small intestinal epithelial cells.
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© 1993 Springer Science+Business Media Dordrecht
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Ametani, A., Hachimura, S., Imaoka, A., Yamamoto, Y., Yi, HK., Kaminogawa, S. (1993). Growth and Differentiation of a Small Intestinal Epithelial Cell Line IEC-6. In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_5
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DOI: https://doi.org/10.1007/978-94-011-2044-9_5
Publisher Name: Springer, Dordrecht
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