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Factors Affecting the Yield of a Baculovirus Insect Cell System by Hollow-fiber Culture

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Animal Cell Technology: Basic & Applied Aspects

Part of the book series: Animal Cell Technology: Basic & Applied Aspects ((ANICELLTECH,volume 5))

Abstract

Directly measurement of the concentration of dissolved oxygen in the medium of a T-flask demonstrated that the oxygen concentration in the medium had a great effect on the virus production. While a high concentration was necessary for the infection process, this was harmful for production yield. A low concentration of oxygen in the production process was required for a high yield of protein.

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References

  1. Smith, G.E., Summers, M.D. and Fraser, M.J. (1983) ‘Production of human beta interferon in insect cells infected with a baculovirus expression vector,’ J. Mol. Cell. Biol. 3 2156–2165

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Author information

Authors and Affiliations

  1. Development Group, Business Development Dept., Tabai Espec Corp., 3-5-6, Tenjinbashi, Kita-ku, Osaka, 530, Japan

    Hideki Shimoizu, Yuko Naka & Nobuyuki Etou

Authors
  1. Hideki Shimoizu
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  2. Yuko Naka
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  3. Nobuyuki Etou
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Editor information

Editors and Affiliations

  1. Department of Agricultural Chemistry, The University of Tokyo, Japan

    S. Kaminogawa , A. Ametani  & S. Hachimura ,  & 

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© 1993 Springer Science+Business Media Dordrecht

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Shimoizu, H., Naka, Y., Etou, N. (1993). Factors Affecting the Yield of a Baculovirus Insect Cell System by Hollow-fiber Culture. In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_48

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  • DOI: https://doi.org/10.1007/978-94-011-2044-9_48

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4905-4

  • Online ISBN: 978-94-011-2044-9

  • eBook Packages: Springer Book Archive

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