Abstract
We have established CD8+ suppressor T cell (Ts) clone 13G2 which produces a suppressive lymphokine, interleukin 10 (IL-10). In order to clarify signal transduction mechanisms of IL-10 production and proliferation of a CD8+ suppressor T cell clone, we examined whether several T cell-specific stimulations could induce both production of IL-10 and proliferation of 13G2 cells. We detected IL-10 production from 13G2 cells stimulated with immobilized monoclonal antibody to CD3/T cell receptor complex molecules or with IL-2. These stimuli induced proliferation of 13G2 cells, while 3H-thymidine uptake by 13G2 cells stimulated with immobilized anti-CD3 was less than that with IL-2. The result from the experiment using hydroxyurea indicates that 13G2 could produce IL-10 without those proliferation, suggesting that the production of IL-10 is unrelated to the proliferation of parental cells.
These results reveal that IL-2 is a good stimulator for both proliferation of Ts and production of IL-10 from Ts.
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© 1993 Springer Science+Business Media Dordrecht
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Minai, Y., Hisatsune, T., Nishijima, K., Enomoto, A., Kaminogawa, S. (1993). Mechanism of IL-10 Production by a CD8+ T Cell Clone. In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_36
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DOI: https://doi.org/10.1007/978-94-011-2044-9_36
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