Abstract
The physiology of a recombinant Chinese hamster ovary cell line in glucose-limited chemostat culture has been studied over a range of dilution rates (D=0.008-0.020h-1). There was a deviation of the specific growth rate (µ) from D at low dilution rates due to an increased specific death rate. Extrapolation of these data suggested a minimum specific growth rate of 0.011h-1 (µmax=0 0.025h-1). The specific rate of glucose uptake increased linearly with µ and KS was calculated to be 59.6µM. There was also a linear increase in the rate of lactate production with a higher yield of lactate from glucose at high growth rates. The specific rate of IFN-γ production increased with µ in a manner indicative of a growth related product. Despite changes in the IFN-γ production rate and cell physiology, the pattern of IFN-γ glycosylation was similar at all except the lowest growth rates where there was a decrease in the proportion of fully glycosylated IFN-γ.
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References
Curling, E.M.A., Hayter, P.M., Baines, A.J., Bull, A.T., Gull, K., Strange, P.G. and Jenkins, N. (1990) Recombinant human interferon-γ: Differences in glycosylation and proteolytic processing lead to heterogeneity in batch culture. Biochem. J. 272, 333–337
Fawcett, J.K. and Scott, J.E. 1960. A rapid and precise method for the dtermination of urea. J. Clin. Pathol. 13, 156–159
Frame, K.K. and Hu, W.-S. 1991. Kinetic study of hybridoma cell growth in continuous culture. I. A model for non-producing cells. Biotechnol. Bioeng. 37, 55–64
Hayter, P.M., Curling, E.M.A., Baines, A.J., Jenkins, N., Salmon, I., Strange, P.G. and Bull, A.T. 1991. Chinese hamster ovary cell growth and interferon production kinetics in stirred batch culture. Appl. Microbiol. Biotechnol. 34, 559–564
Hayter, P.M., Curling E.M.A., Baines, A.J., Jenkins, N., Salmon, I., Strange, P.G., Tong, J.M. and Bull, A.T. 1992. Glucose-limited chemostat culture of Chinese hamster ovary cells producing recombinant human interferon-γ. Biotechnol. Bioeng. 39,327–335
Miller, W.M., Blanch, H.W. and Wilke, C.R. 1988. A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: effect of nutrient concentration, dilution rate and pH. Biotechnol. Bioeng. 32, 947–965
Tovey, M. 1980. The cultivation of animal cells in the chemostat: application to the study of tumor cell multiplication. Adv. Cancer Res. 33, 1–37
Zielke, H.R., Ozand, P.T., Tildon, J.T., Sevdalian, D.A. and Cornblath, M. 1976. Growth of human diploid fibroblasts in the absence of glucose utilization. Proc. Natl. Acad. Sci. USA 73, 4110–4114
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© 1993 Springer Science+Business Media Dordrecht
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Hayter, P.M., Gould, M.L., Salmon, I., Tong, J.M., Bull, A.T. (1993). Chemostat Culture Studies on CHO Cells Producing Interferon-γ. In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_34
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DOI: https://doi.org/10.1007/978-94-011-2044-9_34
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4905-4
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