Abstract
A novel cell line, r/mHM-SFME-1 (r/mHM-1) was established from ras/myc SFME cells transformed by human c-Ha-ras and mouse c-myc genes (SFME cells have been established from a Balb/c mouse embryo in a serum-free culture condition). This cell line was derived from a pulmonary metastasis developed in a Balb/c mouse which had been transplanted subcutaneously with pSV2-neo introduced ras/myc SFME cells. The r/mHM-1 cells had an ability to spontaneously metastasize into the lungs of syngeneic mice when injected subcutaneously, and survival of the mice which received the r/mHM-1 cells was significantly shorter than ones with ras/myc SFME cells. The r/mHM-1 cells grew slowly in vitro than their parental ras/myc SFME ones did, and produced dispersed colonies in agar whereas their parental ones produced packed ones. A urokinase type plasminogen activator activity was detected in the culture fluid in which the r/mHM-1 cells were cultured for 2 days, whereas the activity was not detected in those from the parent ones.
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© 1993 Springer Science+Business Media Dordrecht
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Nomura, T. et al. (1993). Characterization of Newly Established Cells Which Provide an Animal Model for Spontaneous Metastasis. In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_3
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DOI: https://doi.org/10.1007/978-94-011-2044-9_3
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