Abstract
Liver microsomal oxygenases are multicomponent enzyme systems which metabolize a wide variety of xenobiotics. A major component of the system is a group of enzymes collectively known as cytochrome P450 (CP450). A major limitation in the use of rodent hepatocyte cultures in toxicity testing and pharmacokinetic studies has been the rapid loss of phase 1 reactions catalyzed by the CP450-dependent mono-oxygenases. Using a sandwich matrix and a serum-free medium developed by GIBCO, total rat CP450 could be maintained for at least 9 days at 75–80% day “0” levels. Metabolic studies of the microsomal fraction of primary adult rat hepatocytes, measured by the conversion of 7-ethoxycoumarin to 7-hydroxycoumarin and of 3,4-benzo-[a]-pyrene to 3-hydroxybenzo-[a]-pyrene, demonstrated maintenance of activity over the same 9 days comparable to the “0” time controls.
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© 1993 Springer Science+Business Media Dordrecht
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Lobo, J.O., Samrock, R.L., Jayme, D.W., Price, P.J. (1993). Sustained Inducibility of Cytochrome P450 Activity in Rat Hepatocytes Cultured in a Serum-Free Medium. In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_28
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DOI: https://doi.org/10.1007/978-94-011-2044-9_28
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