Abstract
The expression vectors of human erythropoietin (EPO), pZIP-NeoSV(X)1-EPO, and the soluble form of the mouse EPO receptor (sEPO-R), pcmEPR-sol.DHFR, were transfected to HepG2 and CHO-dhfr- cells, respectively, and analyzed with regard to their integration sites on the chromosomes by using fluorescence in situ hybridization (FISH). The integrated DNA was detected by FITC, and FISH images were taken by a cooled CCD digital imaging system connected with a fluorescence microscope. The integrated EPO gene was detected weakly at only one specific site of a given chromosome. On the other hand, the integrated sEPO-R gene gave intense signals because of gene amplification. The integration site of the sEPO-R gene was the telomere region of a pair of specific chromosomes. In interphase nuclei, this gave dispersed signals in contrast to the integrated EPO gene, which gave singlets or doublets.
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© 1993 Springer Science+Business Media Dordrecht
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Okumura, K. et al. (1993). Detection by Fluorescence in Situ Hybridization of the Erythropoietin Gene Introduced into Animal Cells. In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_15
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DOI: https://doi.org/10.1007/978-94-011-2044-9_15
Publisher Name: Springer, Dordrecht
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