Abstract
Nucleotide pool measurements have been shown to be an attractive tool for the control of fermentation processes with recombinant mammalian cells. In particular, the NTP and U values expressed in the NTP/U-plot gives a direct access to the physiological state of the cells. A two times higher perfusion rate had to be adjusted for a sufficent supply to satisfy the physiological requirements of the cells than had been assumed by the use of a classical environmental control.
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© 1993 Springer Science+Business Media Dordrecht
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Valley, U., Ryll, T., Wagner, R. (1993). Process Control of BHK Cells by Nucleotide Pool Measurement. In: Kaminogawa, S., Ametani, A., Hachimura, S. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2044-9_13
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DOI: https://doi.org/10.1007/978-94-011-2044-9_13
Publisher Name: Springer, Dordrecht
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