Skip to main content
SpringerLink
Log in

Detection of antinuclear antibodies by immunofluorescence

  • Chapter
Book cover Manual of Biological Markers of Disease

  • 90 Accesses

Abstract

The indirect immunofluorescence assay (IFA) is the most commonly used technique for the detection of antinuclear antibodies (ANA)[1]. It is a sensitive, reproducible and simple assay that can be performed in almost every laboratory. Because IFA enables the detection of a wide range of autoantibodies, it is generally used as a primary screening test for ANA [2].

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Abbreviations

ANA:

antinuclear antibodies

CIE:

counter-immunoelectrophoresis

hnRNP:

heterogeneous nuclear RNP

IFA:

indirect immunofluorescence assay

JCA:

juvenile chronic arthritis

RA:

rheumatoid arthritis

RNP:

ribonucleoprotein

SLE:

systemic lupus erythematosus

snRNP:

small nuclear RNP

SS:

Sjögren’s syndrome

References

  1. McCarty GA, Valencia DW & Fritzler MJ (1984) Antinuclear antibodies: contemporary techniques and clinical application to connective tissue diseases, pp. 1–96. Oxford Univ. Press., NY.

    Google Scholar 

  2. Fritzler MJ & Tan EM (1985) Antinuclear antibodies and the connective tissue diseases. In: Laboratory diagnostic procedures in rheumatic diseases, (pp. 207–247). Grune & Stratton Edt.

    Google Scholar 

  3. Fritzler MJ (1986) Autoantibody Testing: Procedures and significance in systemic rheumatic diseases. Meth Archiv Exp Pathol 12:224–260. Karger, Basel

    CAS  Google Scholar 

  4. Humbel RL & Kadusch P (1985) Use of HEp-2 cells in the IF test for detection of antinuclear antibodies. Protides of the Biological Fluids, Proc. 35th Colloquium, H. Peeters Edit. Pergamon Press Ldt. 33:385–387

    Google Scholar 

  5. Feltkamp TEW, Klein F & Janssens MBJA (1988) Standardisation of the quantitative determination of antinuclear antibodies (ANAs) with a homogeneous pattern. Ann Rheum Dis 47:906–909

    Article  PubMed  CAS  Google Scholar 

  6. Tan EM, Feltkamp TEW, Alarcon-Segovia D et a1.(1988) Reference reagents for antinuclear antibodies. Arthr Rheum 31:1331

    Article  Google Scholar 

  7. Nakamura RM & Tan EM (1986) Recent advances in laboratory tests and the autoantibodies to nuclear antigens in systemic rheumatic diseases. Rec Adv Immunopath

    Google Scholar 

  8. Krenik KD, Kephart GM, Offord KP, Dunnette SL & Gleich GJ (1989) Comparison of antifading agents used in immunofluorescence. J Immunol Meth 117:91–97

    Article  CAS  Google Scholar 

  9. Fritzler MJ, Pauls JD, Douglas-Kinsella T, Bowen TJ (1985) Anti-nuclear, anticytoplasmic and anti-Sjögren’s syndrome antigen A (SSA/Ro) antibodies in female blood donors. Clin Immunol Immunopathol 36:120–128

    Article  PubMed  CAS  Google Scholar 

  10. Lassoued K, Guilty MN, Danon F, Chantal A, Dhumeaux D, Clauvel J-P, Brouet J-C, Seligmann M & Courvalin J-C (1988) Antinuclear autoantibodies specific for lamins. Ann Int Med 108:829–833

    PubMed  CAS  Google Scholar 

  11. Senecal JL & Raymond Y (1991) Autoantibodies to DNA, lamins and pore complex proteins produce distinct peripheral fluorescent antinuclear antibody patterns on the HEp2 substrate. Arthr Rheum 34:249–251

    Article  CAS  Google Scholar 

  12. Worman HJ & Courvalin JC (1991) Autoantibodies against nuclear envelope proteins in liver disease. Hepatology 14:1270–1279

    Article  Google Scholar 

  13. Tan EM (1982) Autoantibodies to nuclear antigens (ANA): Their immunobiology and medicine. Adv Immunol 33:167–240

    Article  PubMed  CAS  Google Scholar 

  14. Fritzler MJ, Ali R & Tan EM (1984) Antibodies from patients with mixed connective tissue disease react with heterogeneous nuclear ribonucleoproteins or ribonucleic acid (hnRNP/RNA) of the nuclear matrix. J Immunol 132:1216–1222

    PubMed  CAS  Google Scholar 

  15. Van Venrooij WJ & Sillekens PTG (1989) Small nuclear RNA associated proteins: autoantigens in connective tissue diseases. Clin Exp Rheum 7:635–645

    Google Scholar 

  16. Tan EM (1989) Antinuclear antibodies: Diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immun 44:93–152

    Article  PubMed  CAS  Google Scholar 

  17. Bravo R, Frank R, Blundell PA, MacDonald-Bravo H (1987) Cyclin/PCNA is the auxiliary protein of DNA polymerase δ Nature 326:515–517

    Article  PubMed  CAS  Google Scholar 

  18. Andrade LEC, Chan EKL, Raska I, Peebles CL, Roos G & Tan EM (1991) Human autoantibody to a novel protein of the nuclear Coiled Body: Immunological characterization and cDNA cloning of p80-coilin. J Exp Med 173:1407–1419

    Article  PubMed  CAS  Google Scholar 

  19. Szostecki C, Krippner H, Penner E & Bautz FA (1987) Autoimmune sera recognize a 100 kD nuclear protein antigen (Sp-100). Clin Exp Immunol 68:108–116

    PubMed  CAS  Google Scholar 

  20. Bernstein RM, Steigerwald JC & Tan EM (1982) Association of antinuclear and antinucleolar antibodies in progressive systemic sclerosis. Clin Exp Immuno] 48:43–51

    CAS  Google Scholar 

  21. Imai H, Ochs RL, Kiyosawa K, Furuta S, Nakamura RM & Tan EM (1992) Nucleolar antigens and autoantibodies in hepatocellular carcinoma and other malignancies. Am J Pathol 140:859–870

    PubMed  CAS  Google Scholar 

  22. Minota S, Jarjour WN, Suzuki N, Nojima Y, Roubey RAS, Mimura T, Yamada A, Hosoya T, Takaku F & Winfield JB (1991) Autoantibodies to nucleolin in systemic lupus erythematosus and other diseases. J Immunol 146:2249–2252

    PubMed  CAS  Google Scholar 

  23. Blüthner M & Bautz FA (1992) Cloning and characterization of the cDNA coding for a polymyositis-scleroderma overlap syndrome-related nucleolar 100 kDa protein. J Exp Med 176

    Google Scholar 

  24. Okano Y, Steen VD & Medsger TA Jr (1992) Autoantibody to U3 nucleolar ribonucleoprotein (fibrillarin) in patients with systemic sclerosis. Arthritis Rheum 35:95–100

    Article  PubMed  CAS  Google Scholar 

  25. Reimer G, Rose KM, Scheer U & Tan EM (1987) Autoantibody to RNA polymerase I in scleroderma sera. J Clin Invest 79:65–72

    Article  PubMed  CAS  Google Scholar 

  26. Courvalin JC, Hernandez-Verdun D, McCarty MC, Maunoury R & Bornens M (1985) A 80 Kd protein is associated with the nucleolus organizer (NOR) of the human cell lines. Protides of the Biological Fluids, Proc. 35th. Colloquium, H. Peeters Edit. Pergamon Press Ltd, pp. 221–224

    Google Scholar 

  27. Rodriguez JL, Gelpi C, Juarez C & Hardin JA (1987) Anti-NOR 90: A new autoantibody in scleroderma that recognizes a 90-kDa component of the nucleolus-organizing region of chromatin. J Immunol 139:2579–2584

    Google Scholar 

  28. Rattner JB, Martin L, Waisman DM, Johnstone SA & Fritzler MJ (1991) Autoantibodies to the centrosome (centriole) react with determinants present in the glycolytic enzyme enolase. J Immunol 146:2341–2344

    PubMed  CAS  Google Scholar 

  29. Price CM, McCarty GA & Pettijohn DE (1984) NuMA protein is a human autoantigen. Arthr Rheum 27:774–779

    Article  CAS  Google Scholar 

  30. Fritzler MJ, Ayer LM, Gohill J, O’Connor C, Laxer RM & Humbel R-L (1987) An antigen in metaphase chromatin and the midbody of mammalian cells binds to scleroderma sera. J Rheumatol 14:291–294

    PubMed  CAS  Google Scholar 

  31. McCarty GA, Valencia D & Fritzler MJ (1984) Antibody to the mitotic spindle apparatus: Immunologic characterics and cytologic studies. J Rheumatol 11:213–218

    PubMed  CAS  Google Scholar 

  32. Saito E, Yoshimoto Y, Oshima H, Yoshida H & Kinoshita M (1989) Fluorescent antibodies in polymyositis using cultured human skin fibroblasts: Granular perinuclear cytoplasmic staining pattern by sera from patients with polymyositis and pulmonary fibrosis. J Rheumatol 16:47–54

    PubMed  CAS  Google Scholar 

  33. Bonfa E & Elkon KB (1986) Clinical and serologic associations of the antiribosomal P protein antibody. Arthr Rheum 29:981–985

    Article  CAS  Google Scholar 

  34. Mackay IR & Gershwin ME (1989) Molecular basis of mitochondrial autoreactivity in primary biliary cirrhosis. Immuno) Today 10:315–318

    Article  CAS  Google Scholar 

  35. Coppel RL, McNeilage LJ, Surh CD, Van De Water J, Spithill TW, Whittingham S & Gershwin ME (1988) Primary structure of the human M2 mitochondrial autoantigen of primary biliary cirrhosis: Dihydrolipoamide acetyltransferase. Proc Natl Acad Sci USA 85:7317–7321

    Article  PubMed  CAS  Google Scholar 

  36. Reeves WH, Nigam SK & Blobel G (1986) Human autoantibodies reactive with the signal recognition particle. Proc Natl Acad Sci USA 83:9507–9511

    Article  PubMed  CAS  Google Scholar 

  37. Rodriguez JL, Gelpi C, Thompson TM, Real FJ & Fernandez J (1982) Anti-Golgi complex autoantibodies in a patient with Sjögren’s syndrome and lymphoma. Clin Exp Immunol 49:579–586

    PubMed  CAS  Google Scholar 

  38. Kurki P & Vitanen I (1984) The detection of human antibodies against cytoskeletal components. J Imm Methods 67:209–223

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre Hospitalier de Luxembourg, 4, rue Barblé, L-1210, Luxembourg

    R. L. Humbel

Authors
  1. R. L. Humbel
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Nijmegen, The Netherlands

    W. J. van Venrooij

  2. The Mathilda and Terence Kennedy Institute of Rheumatology, London, UK

    R. N. Maini

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Kluwer Academic Publishers

About this chapter

Cite this chapter

Humbel, R.L. (1996). Detection of antinuclear antibodies by immunofluorescence. In: van Venrooij, W.J., Maini, R.N. (eds) Manual of Biological Markers of Disease. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1670-1_2

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-1670-1_2

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-2219-1

  • Online ISBN: 978-94-011-1670-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation