Abstract
A quantitative autoradiographic method has been developed for the in vivo measurement of local cerebral rates for methionine incorporation into proteins in the free-moving rats [1].The accurate measurement of brain protein synthesis, using this model, depends on two main assumptions. The first one is that methionine enters the precursor pool for protein synthesis from the plasma and that unlabeled methionine derived from steady-state protein degradation does not recycle into this pool. The second one is that metabolism of methionine through the transmethylation-transsulfuration pathway is negligible.
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Bobillier, P., Grange, E., Gharib, A., Leclerc, M., Sarda, N., Lepetit, P. (1993). Methionine Metabolism in Rat Brain. In: Mazoyer, B.M., Heiss, W.D., Comar, D. (eds) PET Studies on Amino Acid Metabolism and Protein Synthesis. Developments in Nuclear Medicine, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1620-6_3
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DOI: https://doi.org/10.1007/978-94-011-1620-6_3
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