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Determination of Regional Rates of Cerebral Protein Synthesis in Vivo with L-[1-14C]Leucine as the Tracer Amino Acid

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PET Studies on Amino Acid Metabolism and Protein Synthesis

Part of the book series: Developments in Nuclear Medicine ((DNUM,volume 23))

Abstract

The quantitative autoradiographic L-[1-14C]leucine method for the determination of regional rates of cerebral protein synthesis in vivo takes into account recycling of unlabeled leucine derived from protein degradation into the precursor pool for protein synthesis. We have evaluated the degree of recycling in whole brain by measuring the ratio of the apparent steady state leucine specific activity in the precursor pool (tRNA-bound leucine) to that in arterial plasma. In normal, conscious, adult rats this ratio (λWB) equals 0.58 indicating that 42% of leucine in the precursor amino acid pool is derived from protein breakdown. Evaluation of λi in local brain regions indicates that the degree of recycling does vary regionally. Local rates of leucine incorporation into protein determined with the quantitative autoradiographic technique and regional values of λi ranged from 11.0 in hypoglossal nucleus to 3.8 nmol/g/min in white matter. The average rate in the brain as a whole was found to be 6.1 nmol/g/min. Results of our studies of regeneration in the hypoglossal nucleus and plasticity in the developing monkey visual system suggest that chronic changes in functional activity in a pathway are more likely than acute changes to result in effects on rates of protein synthesis in structures of the pathway.

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Authors
  1. C. Beebe Smith
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Editor information

Editors and Affiliations

  1. Groupe d’Imagerie Neuro-fonctionelle, Service Hospitalier Frédéric Joliot, CEA-DRIPP Orsay, et Hôpital R. Debré, Paris, France

    B. M. Mazoyer

  2. Max-Planck-Institute for Neurological Research, Cologne, Germany

    W. D. Heiss

  3. E.E.C. Concerted Action on PET Investigations of Cellular Regeneration and Degeneration, Service Hospitalier Frédéric Joliot, Hôpital d’Orsay, Orsay, France

    D. Comar

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© 1993 Springer Science+Business Media Dordrecht

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Smith, C.B. (1993). Determination of Regional Rates of Cerebral Protein Synthesis in Vivo with L-[1-14C]Leucine as the Tracer Amino Acid. In: Mazoyer, B.M., Heiss, W.D., Comar, D. (eds) PET Studies on Amino Acid Metabolism and Protein Synthesis. Developments in Nuclear Medicine, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1620-6_2

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  • DOI: https://doi.org/10.1007/978-94-011-1620-6_2

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4706-7

  • Online ISBN: 978-94-011-1620-6

  • eBook Packages: Springer Book Archive

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