Immunochemical methods

  • Miroslav Ferenčík


All immunochemical methods are based on a biospecific linkage between an antigen (hapten) and an antibody, or occasionally, on the non-specific interactions of free or bound antibodies with certain compounds (complement, protein A) or cell receptors (Fc-receptors). Immunochemical methods are used to demonstrate the presence of an antigen or to determine its quantity (when a specific antibody is available) or the quantity of an antibody (when a specific antigen or hapten is available). The course of the antigenantibody reaction is measured by a decrease of one of the pair of reacting components or, more frequently, by the formation of a reaction product, the antigen-antibody complex. Qualitative methods to measure immune complex formation include measurement of turbidity, precipitate, agglutinate or complement fixation. Quantitative methods make it possible to determine the amount of immune complexes produced.


Immune Complex Latex Particle Antigen Concentration Immunochemical Method Antigen Molecule 
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© Springer Science+Business Media Dordrecht 1993

Authors and Affiliations

  • Miroslav Ferenčík
    • 1
  1. 1.Institute of Immunology, Faculty of MedicineComenius UniversityBratislavaCzechoslovakia

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