Abstract
Immunological methods have recently been applied with success to the problems of diagnosis in histopathology. Although pathological anatomy has been a foundation of medical diagnosis for hundreds of years, strengthened over the past century by the addition of microscopy of diseased tissues, a serious deficiency in modern medicine has been the inability of pathologists to ascribe a molecular basis to morphological tissue changes. Because of this, by the 1960s, the reputation which pathologists had built up for their science in medical diagnosis had become diminished. This loss appeared magnified by notable advances in other medical diagnostic fields, notably radiology and biochemistry. Immunology was gaining importance in clinical medicine and, by the early 1970s, sufficient technical expertise had developed in this field for molecular identifications to be made within cells through the specificity of the antigen-antibody combination. In subsequent years, the efficiency of this method was much improved and its application to histopathological diagnosis was now possible. The last ten years have witnessed an outpouring of pathological discoveries utilizing antigen-antibody methods for identification of diagnostic substances in pathological tissues. Pathological changes in the ear, nose and throat have participated in this resurgence. It was fortunate that this came about at a time when clearer ideas had been developed using routine staining methods for the classification of pathological conditions of the ear, nose and throat, as a result of which thenewimmunological methods could be applied to entities whose places in the nosology of otolaryngic diseases were now accepted.
Chapter PDF
References
Polak JM, Van Noorden S. Immunochemistry. Modern methods and applications in pathology and biology. Bristol: Wright; 1986
Coons AH, Leduc EH, Connolly JM. Studies on antibody production. A method for the specific demonstration of specific antibody and its application to the hyperimmune rabbit. J Exp Med. 1955;102:49–60
Marks R, Knight A, Laidler P. Atlas of skin pathology. Lancaster: MTP Press Ltd; 1986
Hyams VJ, Michaels L. Benign adenomatous neoplasms (adenoma) of the middle ear. Clin Otolaryngol. 1976;1:17–26
Mills SE, Fechner RE. Middle ear adenoma. A cytologically uniform neoplasm displaying a variety of architectural patterns. Am J Surg Pathol. 1984;8:677–85
Polak JM, Bloom SR, Endocrine tumours. Edinburgh: Churchill Livingstone; 1985
Krouse JH, Nadol JB, Goodman ML. Carcinoid tumors of the middle ear. Ann Otol Rhinol Laryngol. 1990;99:547–52
Wassef M, Panagiotis K, Polivka M, Nemeth J, Monteil J-P, FrachetBHuy PTB. Middle ear adenoma. A tumor displaying mutinous and neuroendocrine differentiation. Am J Surg Pathol. 1989;13:838–47
Lloyd RV, Blaivas M, Wilson BS. Distribution of chromogranin and S-100 protein in normal and abnormal adrenal medullary tissues. Arch Pathol. 1985;109:633–5
Kliewer KE, Wen D-R, Cancilla PA, Cochran AJ. Paragangliomas: assessment of prognosis by histologic immunohistochemical and ultrastructural techniques. Hum Pathol. 1989;20: 29–39
Weiss MA, Crissman JD. Renal biopsy findings in Wegener’s granulomatosis: segmental necrotizing glomerulonephritis with glomerular thrombosis. Hum Pathol. 1984;15:943–56
van der Woude FJ, Rasmussen N, Lobatto S et al. Autoantibodies against neutrophils and monocytes: tool for diagnosis and marker of disease activity in Wegener’s granulomatosis. Lancet. 1985;1:425–9
Michaels L. Ear, Nose and throat histopathology. London: Springer; 1987
Slootweg L, Lubsen H. Rhabdomyoblasts in olfactory neuroblastoma. Histopathology. 1991;19:182–4
Heffner DK, Hyams VJ. Teratocarcinoma (malignant teratoma?) of the nasal cavity and paranasal sinuses: a clinicopathologic study of 20 cases. Cancer. 1984;53:2140–54
Shanmugaratnam K, ed. Histological typing of upper respiratory tract tumours. 2nd ed. Berlin: Springer; 1991
Gusterson BA, Mitchell DP, Warburton MJ, Carter RC. Epithelial markers in the diagnosis of nasopharyngeal carcinoma. J Clin Pathol. 1983;36:628–31
Thomas JA, Iliescu V, Crawford DH, Ellouz R, Cammoun M, de The G. Expression of HLA-DR antigens in nasopharyngeal carcinoma: an immunohistochemical analysis of the tumour cells and infiltrating lymphocytes. Int J Cancer. 1984;33:813–9
Roholl PJ, de Jong AS, Ramaekers FC. Review. Application of markers in the diagnosis of soft tissue tumours. Histopathology. 1985;9:1019–35
Salisbury J, Isaacson PG. Demonstration of cytokeratins and an epithelial membrane antigen in chordomas and human fetal notochord. Am J Surg Path, 1985;9:791–7
Buchanan G. Infectious mononucleosis. Ear Nose Throat J. 1982;61:557–61
Sosa-Melgarejo J. Vergleichende immunozytochemische Untersuchungen zur rezidivierenden Tonsillitis and Mononucleosis Infectiosa. HNO. 1985;33:75–83
Knowles DM, Jacobiec FA. Cell marker analysis of extranodal lymphoid infiltrates: to what extent does the determination of mono-or polyclonality resolve the diagnostic dilemma of malignant lymphoma v pseudolymphoma in an extranodal site. Sem Diagnos Pathol_ 1985;2:163–8
Lennert K, Feller AC. Histopathology of Non-Hodgkin-lymphomas (according to the updated Kiel-classification). 2nd ed. Berlin: Springer; 1991
Quincy RE, Wells M, Lewis FA, Terry RM, Michaels L, Croft CB. Laryngeal papillomatosis: correlation between severity of disease and prsence of HPV 6 and 11 detected by in situ DNA hybridisation. J Clin Path. 1989;42:694–8
Spencer H. Pathology of the lung, vol. 2. 4th ed. Oxford: Pergamon Press, 1985
Woodruff JM, Huvos AG, Erlandson RA, Shah JP, Gerold FP. Neuroendocrine carcinomas of the larynx. A study of two types, one of which mimics thyroid medullary carcinoma. Am J Surg Pathol. 1985;9:771–90
Wenig BM, Hyams VJ, Heffner DK. Moderately differentiated neuroendocrine carcinoma of the larynx. A clinicopathologic study of 54 cases. Cancer. 1988;62:2658–76
Milroy CM, Rode J, Moss E. Laryngeal paraganglioma and neuroendocrine carcinomas. Histopathology. 1991;18:201–9
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Michaels, L. (1994). Immunocytochemistry in the Diagnosis of Pathological Conditions of the Ear, Nose and Throat. In: Scadding, G.K. (eds) Immunology of ENT Disorders. Immunology and Medicine Series, vol 23. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1436-3_5
Download citation
DOI: https://doi.org/10.1007/978-94-011-1436-3_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4629-9
Online ISBN: 978-94-011-1436-3
eBook Packages: Springer Book Archive