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Gene transfer systems and transposition

  • M. J. Gasson
  • G. F. Fitzgerald

Abstract

The lactic acid bacteria have evolved and been selected for exploitation in a variety of food fermentation processes. The strains currently available have been subject to gene introduction and genetic reorganization by a variety of natural mechanisms. The study of these mechanisms of gene transfer and DNA rearrangement has led to the development of genetic techniques that can be exploited for strain improvement. These systems complement the genetic engineering methods that are described in chapter 2. This chapter covers the characterization and exploitation of conjugation, transduction and transformation in lactic acid bacteria and the exploitation of generalized recombination and chromosomal integration. The lactic acid bacteria harbour a large number of transposable genetic elements and these undoubtedly contribute to the genetic instability that appears characteristic of many strains. The characterization of the known IS elements and the nisin transposon are described as well as the exploitation of heterologous transposons for genetic analysis. The recent development of pulsed field gel electrophoresis for analysis of whole chromosomes together with some elegant genetic strategies has led to progress in physical and genetic mapping of lactococcal genomes which is also reviewed.

Keywords

Lactic Acid Bacterium Electric Field Pulse Lactobacillus Plantarum Lactobacillus Casei Conjugal Transfer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer Science+Business Media Dordrecht 1994

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  • M. J. Gasson
  • G. F. Fitzgerald

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