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Affinity precipitation

  • Chapter
Highly Selective Separations in Biotechnology

Abstract

By tradition, precipitation is a very common unit operation in protein purification. Precipitation can easily be scaled up, since it is based on processes operated on a large industrial scale already. Precipitation is usually based on aggregation caused by some non-specific factor, e.g. hydrophobic interactions, and therefore it is unrealistic to expect any degree of selectivity. Precipitation by additions of salt or organic solvents (Englard and Steifter, 1990), polymer substances (Glatz, 1990), or even through increase in temperature (Englard and Steifter, 1990) has been exploited. If a higher degree of selectivity through the use of affinity interaction within a precipitation method could be introduced, then much would be gained.

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Authors
  1. M. N. Gupta
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  2. B. Mattiasson
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Editors and Affiliations

  1. School of Science & Technology, University of Teesside, Middlesbrough, UK

    G. Street

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Gupta, M.N., Mattiasson, B. (1994). Affinity precipitation. In: Street, G. (eds) Highly Selective Separations in Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1322-9_2

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  • DOI: https://doi.org/10.1007/978-94-011-1322-9_2

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