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Symbiotic Nitrogen Fixation pp 21–29Cite as

Host range, RFLP, and antigenic relationships between Rhizobium fredii strains and Rhizobium sp. NGR234

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Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 57))

Abstract

Rhizobium fredii is a nitrogen-fixing symbiont from China that combines broad host range for nodulation of legume species with cultivar specificity for nodulation of soybean. We have compared 10 R. fredii strains with Rhizobium sp. NGR234, a well known broad host range strain from Papua New Guinea. NGR234 nodulated 16 of 18 tested lugume species, and nodules on 14 of the 16 fixed nitrogen. The R. fredii strains were not distinguishable from one another. They nodulated 13 of the legumes, and in only nine cases were nodules effective. All legumes nodulated by R. fredii were included within the host range of NGR234. Restriction fragment length polymorphisms (RFLPs) were detected with four DNA hybridization probes: the regulatory and common nod genes, nodDABC; the soybean cultivar specificity gene, nolC; the nitrogenase structural genes, nifKDH; and RFRS1, a repetitive sequence from R. fredii USDA257. A fifth locus, corresponding to a second set of soybean cultivar specificity genes, nolBTUVWX, was monomorphic. Using antisera against whole cells of three R. fredii strains and NGR234, we separated the 11 strains into four serogroups. The anti-NGR234 sera reacted with a single R. fredii strain, USDA191. Only one serogroup, which included USDA192, USDA201, USDA217, and USDA257, lacked cross reactivity with any of the others. Although genetic and phenotypic differences among R. fredii strains were as great as those between NGR234 and R. fredii, our results confirm that NGR234 has a distinctly wider host range than R. fredii.

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Abbreviations

RFLP:

restriction fragment length polymorphism

TBS:

tris-buffered saline

YEM:

yeast extract-mannitol

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Authors and Affiliations

  1. Department of Plant Pathology, University of Missouri, Columbia, MO, 65211, USA

    Hari B. Krishnan & Steven G. Pueppke

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  1. Hari B. Krishnan
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  2. Steven G. Pueppke
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P. H. Graham M. J. Sadowsky C. P. Vance

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© 1994 Springer Science+Business Media Dordrecht

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Krishnan, H.B., Pueppke, S.G. (1994). Host range, RFLP, and antigenic relationships between Rhizobium fredii strains and Rhizobium sp. NGR234. In: Graham, P.H., Sadowsky, M.J., Vance, C.P. (eds) Symbiotic Nitrogen Fixation. Developments in Plant and Soil Sciences, vol 57. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1088-4_3

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  • DOI: https://doi.org/10.1007/978-94-011-1088-4_3

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4475-2

  • Online ISBN: 978-94-011-1088-4

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