Abstract
Expression of foreign genes in mammary epithelial cells using milk protein gene promoters was investigated. About 0.5–1.0 kb of the 5′-flanking sequences of bovine αs1-, β-, κ-caseins and a-lactalbumin genes were fused to the CAT gene. The chimeric genes were transfected into a mouse mammary epithelial cell line, HC11, and primary bovine mammary epithelial cells (BMEC) by the calcium phosphate-precipitation method. The HC11 cells transfected with the casein-CAT chimeric genes showed strong CAT activity, whereas very weak or almost no activity was detected in the transfected BMEC. Expression of the casein-CAT chimeric genes in transfected HC11 cells was strongly affected by FBS concentration of the culture medium, though the effect of FBS concentration on the expression of a SV40-CAT chimeric gene was rather weak.
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© 1994 Springer Science+Business Media Dordrecht
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Ahn, JY., Yamamoto, K., Aoki, N., Adachi, T., Nakamura, R., Matsuda, T. (1994). Expression of Milk Protein-Cat Chimeric Genes in Transfected Bovine and Mouse Mammary Epithelial Cells. In: Kobayashi, T., Kitagawa, Y., Okumura, K. (eds) Animal Cell Technology: Basic & Applied Aspects. The Sixth International Meeting of Japanese Association for Animal Cell Technology JAACT’93, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0848-5_39
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DOI: https://doi.org/10.1007/978-94-011-0848-5_39
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