Abstract
We have previously shown that the growth yields for glucose and glutamine are doubled during the exponential growth phase of hybridoma cells, and that the lactate yield from glucose is reduced after the increase in the growth yields. On the other hand, the oxygen uptake rates are unchanged during the whole course of the cultivation. These suggest that the glucose flux to the TCA cycle is enhanced in the late stage of the exponential growth phase, resulting in the decrease in the glutamine flux to the TCA cycle. This paper deals with an energy balance in the protein synthesis including monoclonal antibody production with hybridoma growth before and after the change in the growth yields. The energy balance is considered using ATP regeneration rates in the early and late stages of the exponential growth phase.
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© 1994 Springer Science+Business Media Dordrecht
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Shirai, Y., Kobayashi, A., Yamaguchi, M., Hashimoto, K. (1994). Energy Balance and Protein Synthesis in Hybridoma Culture. In: Kobayashi, T., Kitagawa, Y., Okumura, K. (eds) Animal Cell Technology: Basic & Applied Aspects. The Sixth International Meeting of Japanese Association for Animal Cell Technology JAACT’93, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0848-5_30
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DOI: https://doi.org/10.1007/978-94-011-0848-5_30
Publisher Name: Springer, Dordrecht
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