Abstract
The past five years have introduced a vast technological change in the methodology for histocompatibility testing. The availability of gene probes for the various HLA loci has allowed to study the polymorphisms at these loci at the DNA level using the technique of restriction fragment length polymorphisms. The availability of first allelic sequences together with the new technique of polymerase chain reaction has very quickly lead to a wealth of information on polymorphic sequence of all the classical HLA loci (Marsh and Bodmer, Zemmour and Parham).
Chapter PDF
Similar content being viewed by others
Keywords
- Transcriptional Regulatory Region
- Unrelated Bone Marrow
- Cadaver Kidney Transplant
- Unrelated Bone Marrow Transplantation
- DQA1 Gene
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Andersen LC., Beaty JS., Nettles JW. et al.: Allelic polymorphism in transcriptional regulatory regions of HLA-DQB genes. J. Exp.Med., 1991, 173: 181–192.
Auffray C., Lillie JW., Korman AJ et al.: Structure and expression of HLA-DQα and-DXα genes: interallellic alternate splicing of the HLA-DQα gene and functional splicing of the HLA-DXα gene using a retroviral vector. Immunogenetics 1987, 26: 63–73.
Bein G., Gläser R., Kirchner H.: Rapid HLA-DRB1 genotyping by nested PCR amplification. Tissue Antigens 1992, 39:68–73.
Bidwell J.: DNA-RFLP analysis and genotyping of HLA-DR and-DQ antigens. Immunology today, 1988,9: 18–23.
Cros P., Allibert P., Mandrand B., Tiercy JM. and Mach B.: Oligonucleotide genotyping of HLA polymorphism on microtitre plates. Lancet 1992, 340: 870–873.
Kimura A., Sasazuki T.: Polymorphism in the 52-flanking region of the DQA1 gene and it’s relation to DR-DQ haplotype. In: HLA 1991 (eds Tsuji K., Aizawa M., Sasazuki T.) Vol. II: 382–385, Oxford Sci. Publ. 1993.
Marsh SGE., Bodmer JG.: HLA class II nucleotide sequences 1991. Immunogenetics 1991, 33: 321–334.
Nevinny-Stickel C., Bettinotti MdlP., Andreas A. et al.: Nonradioactive HLA class II typing using polymerase chain reaction and digoxigenin 11-2′-3′-dideoxy-uridinetriphosphate labeled oligonucleotide probes. Hum.Immunol. 1991, 31: 7–13.
Nevinny-Stickel C., Hinzpeter M., Andreas A., Albert ED.: Nonradioactive oligotyping for HLA-DR1-DRw10 using polymerase chain reaction, digoxigenin-labeled oligonucleotides and chemiluminescence detection. Eur.J. Immunogenetics 1991, 18: 323–332.
Olerup O. and Zetterquist H.: HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: An alternative to serological DR typing in clinicaf practice including donor-recipient matching in cadaveric transplantation.
Opelz G., Mytilineos J., Scherer S., Dunckley H., Trejaut J., Chapman J., Middleton D., Savage D., Fischer O., Bignon JD., Bensa JC., Albert E. and Noreen H.: Survival of DNA HLA-DR typed and matched cadaver kidney transplants. The Lancet, 1991, pp. 461–463.
Sherman PA, Basta PV, Ting JPY: Upstream DNA sequences required for tissue specific expression of the HLA-DRα gene. Proc.Natl.Acad.Sci. 1987, 84: 4254–4258.
Singal DP, Qui X., D’Souza M., and Sood SK.: Polymorphism in the upstream regulatory regions of HLA-DRB genes. Immunogenetics 1993, 37:143–147.
Zemmour J. and Parham P.: HLA class I nucleotide sequences, 1992 Europ. J-Immunogenetics. 20:1:29–45.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Albert, E.D. (1994). From Serology to Molecular Histocompatibility Testing. In: Touraine, J.L., Traeger, J., Bétuel, H., Dubernard, J.M., Revillard, J.P., Dupuy, C. (eds) Rejection and Tolerance. Transplantation and Clinical Immunology, vol 25. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0802-7_3
Download citation
DOI: https://doi.org/10.1007/978-94-011-0802-7_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4345-8
Online ISBN: 978-94-011-0802-7
eBook Packages: Springer Book Archive