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Video and microorganisms

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Book cover Video Techniques in Animal Ecology and Behaviour

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Abstract

The greatest difference between motion recording systems, such as cinematography and video recording, and conventional picture recording using microscopic photography, is the acquisition of a time dimension. Temporal data is, of course, very important in behavioural research. Nowadays, behavioural investigations, especially into organellar motility, are needed also in the taxonomic or morphological sciences for microorganisms (Inouye and Hori, 1991). For taxonomic studies on the evolution of protists, for example, morphological studies are not sufficient: when the research objects become more primitive, motile specificity should also be considered. Even for physiological research into protozoa there is insufficient ethological observation (Ricci, 1990).

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References

  • Allen, R.D. and Allen, N.S. (1981) Videomicroscopy in the study of protoplasmic streaming and cell movement. Protoplasma, 109, 209–216.

    Article  Google Scholar 

  • Allen, R.D. and Allen, N.S. (1983) Video-enhanced microscopy with a computer frame memory. Journal of Microscopy, 129(1), 3–17.

    Article  PubMed  CAS  Google Scholar 

  • Allen, R.D., Allen, N.S. and Travis, J.L. (1981) Video-enhanced contrast, differential interference contrast (AVEC-DIC) microscopy: a new method capable of analyzing microtubule-related motility in the reticulopodial network of Allogromia laticollaris. Cell Motility, 1, 291–302.

    Article  CAS  Google Scholar 

  • Baba, S.A., Inomata, S., Ooyama, M. et al. (1991) Three-dimensional recording and measurement of swimming paths of microorganisms with two synchronized monochrome cameras. Review of Scientific Instrumentation, 62(2), 540–541.

    Article  Google Scholar 

  • Baba, S.A. and Mogami, Y. (1985) An approach to digital image analysis of bending shapes of eukaryotic flagella and cilia. Cell Motility, 5, 475–489.

    Article  Google Scholar 

  • Baba S.A. and Mogami, Y. (1987) Device for controlled intensification of the light output of xenon flash tubes. Review of Scientific Instrumentation, 58(7), 1312–1313.

    Article  Google Scholar 

  • Brokaw, C.J. (1965) Non-sinusoidal bending waves of sperm flagella. Journal of Experimental Biology, 43, 155–169.

    PubMed  CAS  Google Scholar 

  • Brokaw, C.J. (1979) Calcium-induced asymmetrical beating of Triton-demem-branated sea urchin sperm flagella. Journal of Cell Biology, 82, 401–411.

    Article  PubMed  CAS  Google Scholar 

  • Brokaw, C.J. (1983) The constant curvature model for flagellar bending patterns. Journal of Submicroscopic Cytology, 15(1), 5–8.

    Google Scholar 

  • Brokaw, C.J. (1985) Computer simulation of flagellar movement. VI. Simple curvature-controlled models are incompletely specified. Biophysics Journal, 48, 633–642.

    Article  CAS  Google Scholar 

  • Brokaw, C.J. (1990) Computerized analysis of flagellar motility by digitization and fitting of film images with straight segments of equal length. Cell Motility and Cytoskeleton, 17(4), 309–316.

    Article  CAS  Google Scholar 

  • Brokaw, C.J. and Kamiya, R. (1987) Bending patterns of Chlamydomonas flagella: IV. Mutants with defects in inner and outer dynein arms indicate differences in outer dynein arm function. Cell Motility and Cytoskeleton, 8, 68–75.

    Article  CAS  Google Scholar 

  • Brokaw, C.J. and Luck, D.J.L. (1985) Bending patterns of Chlamydomonas flagella: III. A radial spoke head deficient mutant and a central pair deficient mutant. Cell Motility, 5, 195–208.

    Article  PubMed  CAS  Google Scholar 

  • Brundage, R.A., Fogarty, K.E., Tuft, R.A. and Fay, F.S. (1991) Calcium gradients underlying polarization and Chemotaxis of eosinophils. Science, 254, 703–706.

    Article  PubMed  CAS  Google Scholar 

  • de Peyer, J.E. and Machemer, H. (1977) Membrane excitability in Stylonychia: Properties of the two-peak regenerative Ca-response. Journal of Comparative Physiology, 121, 15–32.

    Article  Google Scholar 

  • Deitmer, J.W., Machemer, H. and Martinac, B. (1983) Simultaneous recording of responses of membranelles and cirri in Stylonychia under membrane voltage-clamp. Journal of Submicroscpic Cytology, 15(1), 285–288.

    Google Scholar 

  • Donovan, R.M., Goldstein, E., Kim, Y. et al. (1986) A quantitative method for the analysis of cell shape and locomotion. Histochemistry, 84(4–6), 525–529.

    Article  PubMed  CAS  Google Scholar 

  • Dusenbery, D.B. (1985) Using a microcomputer and video camera to simultaneous track 25 animals. Computers in Biology and Medicine 15(4), 169–175.

    Article  PubMed  CAS  Google Scholar 

  • Dworkin, M. (1983) Tactic behavior of Myxococcus xanthus. Journal of Bacteriology, 154(1), 452–459.

    PubMed  CAS  Google Scholar 

  • Eckert, R. (1972) Bioelectric control of ciliary activity. Science, 176, 473–481.

    Article  PubMed  CAS  Google Scholar 

  • Eckert, R. and Sibaoka, T. (1967) Bioelectric regulation of tentacle movement in a dinoflagellate. Journal of Experimental Biology, 47, 433–466.

    PubMed  CAS  Google Scholar 

  • Eggersdorfer, B. and Häder, D.-P. (1991) Phototaxis, gravitaxis and vertical migrations in the marine dinoflagellate Prorocentrum micans. FEMS Microbiology and Ecology, 85, 319–326.

    Article  Google Scholar 

  • Eshel, D. and Gibbons, I.R. (1989) External mechanical control of the timing of bend initiation in sea urchin sperm flagella. Cell Motility and Cytoskeleton, 14, 416–423.

    Article  CAS  Google Scholar 

  • Ettienne, E.M. (1970) Control of contractility in Spirostomum by dissociated calcium ions. Journal of General Physiology, 56, 168–179.

    Article  PubMed  CAS  Google Scholar 

  • Gibbons, L.R., Shingyoji, C., Murakami, A. and Takahashi, K. (1987) Spontaneous recovery after experimental manipulation of the plane of beat in sperm flagella. Nature, 325(6102), 351–352.

    Article  PubMed  CAS  Google Scholar 

  • Häder, D.-P. and Lebert, M. (1985) Real time computer-controlled tracking of multi microorganisms. Photochemistry and Photobiology, 42(5), 509–514.

    Article  PubMed  Google Scholar 

  • Häder, D.-P. and Poff, K.L. (1979) Light-induced accumulation of Dictyosterium discoideum amoebae. Photochemistry and Photobiology, 29(6), 1157–1162.

    Article  Google Scholar 

  • Hasegawa, K., Tanakadate, A. and Ishikawa, H. (1988) A method for tracking the locomotion of an isolated microorganism in real time. Physiology and Behaviour, 42(4), 397–400.

    Article  CAS  Google Scholar 

  • Hou, C.L. and Brücke, E. Th. (1931) Reizversuche an Vorticellen (Alles-oder-nichts-Gesatz, Dekrement der Erregungsleitung in der Narkose, Chronaxie). Pflügers Archiv, 226, 411–417.

    Article  Google Scholar 

  • Inoué, S. (1981) Video image processing greatly enhances contrast, quality, and speed in polarization-based microscopy. Journal of Cell Biology, 89, 346–356.

    Article  PubMed  Google Scholar 

  • Inouye, I., and Hori, T. (1991) High-speed video analysis of the flagellar beat and swimming patterns of algae: possible evolutionary trends in green algae. Protoplasma; 164, 54–69.

    Article  Google Scholar 

  • Inouye, I., Hori, T. and Chihara, M. (1990) Absolute configuration analysis of the flagellar apparatus of Pterosperma cristatum (Prasinophyceae) and consideration of its phylogenetic position. Journal of Phytology, 26, 329–344.

    Article  Google Scholar 

  • Inouye, I. and Pienaar, R.N. (1984) Light and electron microscope observations on Nephroselmis astigmatica sp. nov. (Class Prasinophyceae). Nordic Journal of Botany, 4, 409–423.

    Article  Google Scholar 

  • Iwatsuki, K. and Naitoh, Y. (1981) The role of symbiotic Chlorella in photo-response of Paramecium bursaria. Proceedings of the Japanese Academy, 57(B8), 318–323.

    Article  Google Scholar 

  • Iwatsuki, K. and Naitoh, Y. (1983) Behavioral responses in Paramecium micronucleatum to visible light. Photochemistry and Photobiology, 37(4), 415–419.

    Article  Google Scholar 

  • Izumi-Kurotani, A., Yamashita, M., Mogami, Y. and Baba, S.A. (1989) Three-dimensional swimming behaviour of Paramecium under various gravitational environments. Zoological Science, 6(6), 1089.

    Google Scholar 

  • Katz, D.F. and Overstreet, J.W. (1981) Sperm motility assessment by video-micrography. Fertility and Sterility, 35(2), 188–193.

    PubMed  CAS  Google Scholar 

  • Kawachi, M., Inouye, I., Maeda, O. and Chihara, M. (1991). The haptonema as a food-capturing device: observation on Chrysochromulina hirta (Prym-nesiophyceae). Phycologia, 30(6), 563–573.

    Article  Google Scholar 

  • Kinosita, H., Murakami, A. and Yasuda, M. (1965) Interval between membrane potential change and ciliary reversal in Paramecium immersed in Ba-Ca mixture. Journal of the Faculty of Science of the University of Tokyo, IV, 10(3), 421–425.

    Google Scholar 

  • Machemer, H. (1974) Frequency and directional response of cilia to membrane potential change in Paramecium. Journal of Comparative Physiology, 92, 293–316.

    Article  Google Scholar 

  • Machemer, H. (1976) Interactions of membrane potential and cations in regulation of ciliary activity in Paramecium. Journal of Experimental Biology, 65, 427–448.

    PubMed  CAS  Google Scholar 

  • Machemer, H. (1977) Motor activity and bioelectric control of cilia. Fortschritte der Zoologie, 24(2/3), 196–210.

    Google Scholar 

  • Machemer, H. (1986) Electromotor coupling in cilia. Fortschritte der Zoologie, 33, 205–250.

    CAS  Google Scholar 

  • Machemer, H. and Eckert, R. (1975) Ciliary frequency and orientational responses to clamped voltage step in Paramecium. Journal of Comparative Physiology, 104, 247–260.

    Article  Google Scholar 

  • Machemer, H., Machemer-Röhnisch, S., Bräucker, R. and Takahashi, K. (1991) Gravikinesis in Paramecium: theory and isolation of a physiological response to the natural gravity vector. Journal of Comparative Physiology A, 168, 1–12.

    Article  Google Scholar 

  • Machemer, H. and Sugino, K. (1989) Electrophysiological control of ciliary beating: a basis of motile behaviour in ciliate protozoa. Comparative Biochemistry and Biophysics, 94A, 365–374.

    Google Scholar 

  • Maier, I. and Müller, D.G. (1990) Chemotaxis in Laminaria digitata (Phaeophyceae) I. Analysis of spermatozoid movement. Journal of Experimental Botany, 41(228), 869–876.

    Article  Google Scholar 

  • Matsumoto, G. and Ichikawa, M. (1990) Optical system for real-time imaging of electrical activity with a 128 × 128 photopixel array. Society of Neuroscience Abstracts, 16, 490.

    Google Scholar 

  • Moreton, R.B. and Amos, W.B. (1979) Electrical recording from the contractile ciliate Zoothamnium geniculatum Ayrton. Journal of Experimental Biology, 83, 159–167.

    Google Scholar 

  • Naitoh, Y. (1959) Relation between the deformation of the cell membrane and the change in beating direction of cilia in Opalina. Journal of the Faculty of Science of the University of Tokyo, IV, 8(3), 357–369.

    Google Scholar 

  • Naitoh, Y. (1966) Reversal response elicited in nonbeating cilia of Paramecium by membrane depolarization. Science, 154(3749), 660–662.

    Article  PubMed  CAS  Google Scholar 

  • Naitoh, Y. and Eckert, R. (1969) Ciliary orientation: controlled by cell membrane or by intracellular fibrils? Science, 166, 1633–1635.

    Article  PubMed  CAS  Google Scholar 

  • Naitoh, Y. and Kaneko, H. (1972) Reactivated Triton-extracted models of Paramecium: modification of ciliary movement by calcium ions. Science, 176, 523–524.

    Article  CAS  Google Scholar 

  • Naitoh, Y. and Sugino, K. (1984) Ciliary movement and its control in Paramecium. Journal of Protozoology, 31, 31–40.

    Google Scholar 

  • Nakazato, H. and Naitoh, Y. (1980) Factors determining the chemotactic behaviour in Paramecium. Zoological Magazine (Tokyo), 89(4), 434.

    Google Scholar 

  • Nakazato, H. and Naitoh, Y. (1981) Chemokinesis in Paramecium caudatum. Zoological Magazine (Tokyo), 90(4), 677.

    Google Scholar 

  • Nakazato, H. and Naitoh, Y. (1982) Chemokineses in Paramecium (II) Response to ‘attractant’ and ‘repellant’. Zoological Magazine (Tokyo), 91(4), 422.

    Google Scholar 

  • Nelson, G.A., Roberts, T.M. and Ward, S. (1982) Caenorhabditis elegans spermato-zoan locomotion. Amoeboid movement with almost no actin. Journal of Cell Biology, 92, 121–131.

    Article  PubMed  CAS  Google Scholar 

  • Nichols, K.M. and Rikmenspoel, R. (1977) Mg2 +-dependent electrical control of flagellar activity in Euglena. Journal of Cell Science, 23, 211–225.

    PubMed  CAS  Google Scholar 

  • Okajima, A. and Kinosita, H. (1966) Ciliary activity and coordination in Euplotes eurystomus — I. Effect of microdissection of neuromotor fibres. Comparative Biochemistry and Physiology, 19, 115–131.

    Article  Google Scholar 

  • Okajima, A. (1953) Studies on the metachronal wave in Opalina I. Electrical stimulation with the micro-electrode. Japanese Journal of Zoology, 11, 87–100.

    Google Scholar 

  • Reize, L.B. and Melkonian, M. (1989) A new way to investigate living flagellated/ciliated cells in the light microscope: immobilization of cells in agarose. Botanica Acta, 102(2), 145–151.

    Google Scholar 

  • Ricci, N. (1990) The behaviour of ciliated protozoa. Animal Behaviour, 40, 1048–1069.

    Article  Google Scholar 

  • Rüffer, U. and Nultsch, W. (1987) Comparison of the beating of eis- and trans-flagella of Chlamydomonas cell held on micropipettes. Cell Motility and Cytoskeleton, 7, 87–93.

    Article  Google Scholar 

  • Russo, A., Gualtieri, P. and Ricci, N. (1988) A semiautomatic computerized analysis of tracks of ciliates. Experientia, 44, 277–280.

    Article  Google Scholar 

  • Satir, P. (1965) Studies on cilia II. Examination of the distal region of the ciliary shaft and the role of the filaments in motility. Journal of Cell Biology, 26, 805–834.

    Article  PubMed  CAS  Google Scholar 

  • Shingyoji, C., Gibbons, L.R., Murakami, A. and Takahashi, K. (1991) Effect of imposed head vibration on the stability and wave-form of flagellar beating in sea-urchin spermatozoa. Journal of Experimental Biology, 156, 63–80.

    PubMed  CAS  Google Scholar 

  • Sleigh, M.A. (ed) (1974) Cilia and Flagella, Academic Press, London.

    Google Scholar 

  • Sugino, K. and Machemer, H. (1987) Axial-view recording: an approach to assess the third dimension of the ciliary cycle. Journal of Theoretical Biology, 125, 67–82.

    Article  Google Scholar 

  • Sugino, K. and Machemer, H. (1988) The ciliary cycle during hyperpolarizationinduced activity: an analysis of axonemal function parameters. Cell Motility and Cytoskeleton, 11, 275–290.

    Article  CAS  Google Scholar 

  • Sugino, K. and Machemer, H. (1990) Depolarization controlled parameters of the ciliary cycle and axonemal function. Cell Motility and Cytoskeleton, 16, 251–265.

    Article  Google Scholar 

  • Sugino, K. and Naitoh, Y. (1988) Estimation of ciliary activity in Paramecium from its swimming path. Seitai no Kagaku, 39(5), 485–490.

    Google Scholar 

  • Tuenis, P.F.M., Bretschneider, F. and Machemer, H. (1992). Real-time three-dimensional tracking of fast-moving microscopic objects. Journal of Microscopy (Oxford), 168(3), 275–288.

    Article  Google Scholar 

  • Throndsen, J. (1988) Cymbomonas Sciller (Prasinophyceae) reinvestigated by light and electron microscopy. Archiv für Protistenkunde, 136, 327–336.

    Article  Google Scholar 

  • Tieman, D.G., Murphey, R.K., Schmidt, JT. and Tieman, S.B. (1986) A computer-assisted video technique for preparing high-resolution pictures and stereograms from thick specimens. Journal of Neuroscience Methods, 17, 231–245.

    Article  PubMed  CAS  Google Scholar 

  • Tominaga, T. and Naitoh, Y. (1992) Membrane potential responses to thermal stimulation and the control of thermoaccumulation in Paramecium caudatum. Journal of Experimental Biology (in press).

    Google Scholar 

  • Turano, T.A., D’Arpa, P., Clark, W.L. and Williams, J.R. (1985) A time-lapse, image digitization videomicroscope system based on a minicomputer with large peripheral memory. Computers in Biology and Medicine, 15(4), 177–185.

    Article  PubMed  CAS  Google Scholar 

  • Van Houten, J., Hansma, H. and Kung, C. (1975) Two quantitative assays for chemotaxis in Paramecium. Journal of Comparative Physiology, 104, 211–223.

    Article  Google Scholar 

  • Van Houten, J., Hauser, D.C.R. and Levandowsky, M. (1981) Chemosensory behavior of protozoa, in Biochemistry and Physiology of Protozoa 4 (eds M. Levandowsky and S. Hutner), Academic Press, New York, pp. 67–125.

    Google Scholar 

  • Wood, D.C. (1970) Electrophysiological studies of the protozoan, Stentor coeruleus. Journal of Neurobiology, 1(4), 363–377.

    Article  PubMed  CAS  Google Scholar 

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  1. K. Sugino
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Editors and Affiliations

  1. Department of Entomology and Animal Ecology, Lincoln University, New Zealand

    Stephen D. Wratten

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© 1994 Springer Science+Business Media Dordrecht

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Sugino, K. (1994). Video and microorganisms. In: Wratten, S.D. (eds) Video Techniques in Animal Ecology and Behaviour. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0699-3_9

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  • DOI: https://doi.org/10.1007/978-94-011-0699-3_9

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