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Iron Nutrition in Soils and Plants pp 247–254Cite as

The influence of the iron content on the superoxide dismutase activity and chloroplast ultrastructure of Citrus limon

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Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 59))

Abstract

The subject of this study is the relationship between iron content, superoxide dismutase activity and chloroplast ultrastructure in Citrus limon leaves affected by lime-induced iron chlorosis. Iron deficiency led to important decreases in chlorophyll levels, subcellular iron fractions and superoxide dismutase (SOD) activity, especially Fe-SOD isozymes. Iron resupply to chlorotic leaves brought about the restoration of the iron levels, SOD activity and chloroplast ultrastructure which became similar to those shown by control green leaves.

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References

  • Abadía J 1992 Leaf responses to Fe deficiency: A review. J. Plant Nutr. 15, 1699–1713.

    Article  Google Scholar 

  • Alcaraz C F, Martínez-Sánchez F, Sevilla F and Hellín E 1986 Influence of ferredoxin levels on nitrate activity in iron deficient lemon leaves. J. Plant Nutr. 9, 1405–1413.

    Article  CAS  Google Scholar 

  • Almansa M S, Del Rio L A, Alcaraz C F and Sevilla F 1989 Isoenzyme pattern of superoxide dismutase in different varieties of Citrus plants. Pyshiol. Plant. 76, 563–568.

    Article  CAS  Google Scholar 

  • Bogorad L G, Switf H and Mcllrath W J 1959 The structure of chloroplast in leaf tissue of iron deficient Xantium. Brookhaven Symp.Biol. 11, 132–137.

    Google Scholar 

  • Bowler C H, Van Montagu M and Inzé D 1992 Superoxide dismutase and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43, 83–116.

    Article  CAS  Google Scholar 

  • Bradford M M 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 275–278.

    Article  Google Scholar 

  • Bridges S M and Salin ML 1981 Distribution of iron containing superoxide dismutase in vascular plants. Plant Physiol. 68, 274–278.

    Article  Google Scholar 

  • Carpena O, Sierra F, Ortuno A, Monllor E, Parra M and Alcaraz C F 1967 Nuevas Orientaciones en el Diagnostico y Tratamiento de las Deficiencias de Hierro y Manganeso. CEBAS-CSIC, Murcia, Spain. 168p.

    Google Scholar 

  • Del Amor F, León A and Torrecillas A 1985 Guía Práctica para el Riego y la Fertilizacíon de los Cítricos. C.R.C.S. Coop, de Cdto. Ltda. Murcia. 2nd, Murcia, Spain. 109p.

    Google Scholar 

  • Del Río L A, Sandalio L M, Plama J M, Bueno P and Corpas F L 1992 Metabolism of oxygen radicals in peroxisomes and cellular implications. Free Rad. Biol. Med. 13, 557–580.

    Article  PubMed  Google Scholar 

  • Del Río L A, Sevilla F, Sandalio L M and Palma J M 1991 Nutritional effect and expression of superoxide dismutases: induction and gene expression: diagnostic; prospective protection against oxygen toxicity. Free Rad. Res. Comm. 12–13, 819–827.

    Article  Google Scholar 

  • Elstner E F, Wagner G A and Shutz W 1988 Activate oxygen in green plants in relation to stress situations. Curr. Top. Plant Biochem. Physiol. 7, 159–187.

    Google Scholar 

  • Elstner E F 1990 Der Sauerstoff, Biochimie, Biologie, Medizin. B I Wissenschaftsverlag, Mannheim.

    Google Scholar 

  • Fernández-López J A 1991 Determinacíon de pigmentos fotosintéticos y caracterización del enzima clorofilasa de hojas de C. limon deficientes en hierro. Evolucíon con el grado de cloro-sis. Tesis doctoral. Universidad de Murcia. 222p.

    Google Scholar 

  • Fridovich I 1986 Biological effects of the superoxide radicals. Arch. Biochem. Biophys. 247, 1–11.

    Article  PubMed  CAS  Google Scholar 

  • Hall J D, Barr R, Al-Abbas A H and Crane F L 1972 The ultrastructure of chloroplast in mineral-deficient maize leaves. Plant Physiol. 50, 404–409.

    Article  PubMed  CAS  Google Scholar 

  • Halliwell B and Gutteridge J M C 1986 Free radicals in Biology and Medicine. 2nd ed. Clarendon Press, Oxford. 543 p.

    Google Scholar 

  • Hech-BuchholzC H 1983 Light and electron microscopic investigations of the reactions of various genotypes to nutritional disorders. Plant and Soil 72, 151–165.

    Article  Google Scholar 

  • Hellin E, Llorente S, Piquer V and Sevilla S 1984 Actividad perox-idasa inducida como indicador de la efectividad de compuestos orgánicos de hierro en la correcion de la clorosis Fe en el limonero. Agrochimica XXVIII, 432–441.

    Google Scholar 

  • Hellin E, Ureña R, Sevilla F and Alcaraz C F 1987 Comparative study on the effectiveness of several iron compounds in the iron chlorosis correction in Citrus plants. J. Plant Nutr. 10, 411–421.

    Article  CAS  Google Scholar 

  • Hellin E, Piqueras A, Sevilla F, Alcaraz C F and Agulleiro B 1988 Influenciade la concentración foliar de hierro sobre la utraestructura de los cloroplastos de hojas de limonero Verna. In Nutrition Mineral de las Plantas, pp 257–265. I.C.E. Universidad de Granada.

    Google Scholar 

  • Hewitt E J 19XX Essential and functional metals in plants. In Metals and Micronutrients: Uptake and Utilization by Plants. Eds. D A Robb and W S Pierpoint, pp 277–323. Academic Press, NY.

    Google Scholar 

  • Inskeep P W and Bloom P R 1985 Extinction coefficients of chlorophyll a and b in N,N’-dimethylformamideand 80% acetone. Plant Physiol. 77, 483–485.

    Article  PubMed  CAS  Google Scholar 

  • Machold O 1971 Lamellar proteins of green and chlorotic chloroplast affected by iron deficiency and antibiotics. Biochim. Biophys. Acta 238, 324–331.

    Article  PubMed  CAS  Google Scholar 

  • McCord J M and Fridovich I 1969 Superoxide dismutase: An enzymic function for erithrocuprein. J. Biol. Chem. 244, 6049–6055.

    PubMed  CAS  Google Scholar 

  • Nishio J N, Taylor S E and Terry N 1985 Changes in thylakoid galac-tolipids and proteins during iron nutrition-mediated chloroplast development. Plant Physiol. 77, 705–711.

    Article  PubMed  CAS  Google Scholar 

  • Olmos E, Piqueras A and Hellin E 1992 Cytological changes related with salt tolerance in cultured cells of Citrus limon. In Electron Microscopy. Eds. L Megías-Megías, M I Rodríguez-García, A Ríos and Jm Arias. Vol III. EUREM. Secretariado de publica-ciones Universidad de Granada, pp 461–462.

    Google Scholar 

  • Ortiz J M 1980 Nomenclatura botánica de los citricos. Fruits 41, 199–209.

    Google Scholar 

  • Platt-Aloia K A, Thomson W W and Terry N 1983 Changes in plastid ultrastructure during iron nutrition-mediated chloroplast development. Protoplasma 114, 85–92.

    Article  Google Scholar 

  • Price C A 1968 Iron compounds and plant nutrition. Annu. Rev. Plant Physiol. 19, 239–248.

    Article  CAS  Google Scholar 

  • Pugh S Y R and Fridovich I 1985 Induction of superoxide dismutases in Escherichia coliby metal chelators. J. Biochem. 162, 192–202.

    Google Scholar 

  • Pushik J C and Miller G W 1982 The effects of iron and light treatment on chloroplast composition and ultrastructure in iron-deficient barley leaves. J. Plant. Nutr. 5, 311–321.

    Article  Google Scholar 

  • Quílez R and Abadía J 1992 Characteristics of thylakoids and photosystem II membrane preparation from iron deficient and iron sufficient sugar beet (Beta vulgaris L.). J. Plant. Nutr. 15, 1809–1819.

    Article  Google Scholar 

  • Salin M L 1987 Toxic oxygen species and protective system of the chloroplast. Physiol. Plant. 72, 681–689.

    Article  Google Scholar 

  • Seckback J 1982 Ferreting out; the secrets of plant ferritin. A review. J. Plant Nutr. 5, 369–394.

    Article  CAS  Google Scholar 

  • Sevilla F, Hellin E and Del Río L A 1984 Superoxide dismutases from a Citrus plant: Presence of two iron-containing isoenzymes in leaves of lemon trees. J. Plant Physiol. 116, 381–387.

    Article  PubMed  CAS  Google Scholar 

  • Sevilla F, Hellín E, Alcaraz C F and Del Río L A 1987 Induction of iron and manganese superoxide dismutases in Citrus limonum leaves by iron. Plant Physiol. (Life Sci. Adv.) 6, 133–139.

    Google Scholar 

  • Spiller S and Terry N 1980 Limiting factors in photosynthesis. II. Iron stress diminishes photochemical capacity by reducing the number of photosynthetic units. Plant Physiol. 65, 121–125.

    Article  PubMed  CAS  Google Scholar 

  • Stocking C R 1975 Iron deficiency and the structure and physiology of maize chloroplast. Plant Physiol. 55, 626–631.

    Article  PubMed  CAS  Google Scholar 

  • Terry N 1980 Limiting factors in photosynthesis I. Use of iron stress to control photochemical capacity in vivo. Plant Physiol. 65, 114–120.

    Article  PubMed  CAS  Google Scholar 

  • Terry N and Low G 1982 Leaf chlorophyll content and its relation to the intracellular localization of iron. J. Plant Nutr. 5, 301–310.

    Article  CAS  Google Scholar 

  • Terry N 1983 Limiting factors in photosynthesis. IV. Iron stressmediate changes in light harvesting and electron transport capacity and its effects on photosynthesis in vivo. Plant Physiol. 71, 855–860.

    Article  PubMed  CAS  Google Scholar 

  • Terry N and Abadía J 1986 Function of iron in chloroplast. J. Plant Nutr. 9, 609–646.

    Article  CAS  Google Scholar 

  • Watley J M 1971 Ultrastructural changes in chloroplast of Phaseolus vulgaris during development under conditions of nutrient deficiency. New Phytol. 70, 725–742.

    Article  Google Scholar 

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Authors and Affiliations

  1. U.E.I. n°3 CEBAS-CSIC, Av. de la Fama 1, P.O. Box 4195, 30080, Murcia, Spain

    E. Hellín, J.A. Hernández-Cortés, A. Piqueras, E. Olmos & F. Sevilla

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  1. E. Hellín
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  2. J.A. Hernández-Cortés
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  3. A. Piqueras
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  4. E. Olmos
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  5. F. Sevilla
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J. Abadía

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© 1995 Springer Science+Business Media Dordrecht

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Hellín, E., Hernández-Cortés, J., Piqueras, A., Olmos, E., Sevilla, F. (1995). The influence of the iron content on the superoxide dismutase activity and chloroplast ultrastructure of Citrus limon . In: Abadía, J. (eds) Iron Nutrition in Soils and Plants. Developments in Plant and Soil Sciences, vol 59. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0503-3_36

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  • DOI: https://doi.org/10.1007/978-94-011-0503-3_36

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4224-6

  • Online ISBN: 978-94-011-0503-3

  • eBook Packages: Springer Book Archive

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