Abstract
A perfusion bioreactor system has been developed for independent control of cell density and metabolite concentrations. The cell density was monitored and controlled using an online optical sensor. A constant environment to the cell was provided by controlling metabolite concentrations. This was accomplished by altering the perfusion rate using a knowledge-based process control algorithm and on-line metabolic rate measurements. The system is controlled by an object oriented computer program. This high cell density bioreactor permitted the investigation of mammalian cell growth, metabolism and product kinetics at cell concentrations of 0.1 to 20 million cells/mL. Experimental data on the effects of cell density on cell growth, metabolism and productivity under a constant cellular environment (pH, temperature, dissolved oxygen and medium composition) obtained in this reactor are discussed.
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© 1995 Springer Science+Business Media Dordrecht
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Ozturk, S.S., Blackie, J., Wu, P., Figueroa, C., Thrift, J., Naveh, D. (1995). Investigation of High Density Cell Culture Kinetics Using On-Line Cell Density and Metabolic Rate Measurements. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_47
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DOI: https://doi.org/10.1007/978-94-011-0437-1_47
Publisher Name: Springer, Dordrecht
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