Abstract
A replication deficient adenovirus containing the measles virus N gene (nucleoprotein) was propagated on a specially constructed mammalian helper cell line (293), and used to infect a target cell population (MRC5) where expression of the cloned gene only is obtained from the strong CMV IE promoter. Electron microscopy showed that the N protein accumulates in both the cytoplasm and nucleus of infected cells. The growth of 293 cells was compared in a variety of cell culture systems including static flasks, cell aggregates and a variety of microcarriers. The best results were achieved using the Fibra-cel carrier. Virus yields were comparable with the Fibra-cel carriers, cell aggregates and static flasks.
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References
Rima BK (1989)J Gen Micro 64:1205–1219.
Drillien R, Spehner D, Kim A, Giraudon P, Buckland R, Wild F, Lecocq JP (1988) Proc Natl Acad Sci, USA 87:638–652.
Bankamp B, Brinkmann UG, Reich A, Niewiesk S, ter Meulen V, Liebert UG (1991)J Virol 65:1695–1700.
Spehner D, Drillien R, Lecocq JP (1990)J Virol 64:527–533.
Taylor J, Wienberg R, Tartaglia J, Richardson C, Alkhatib G, Briedis D, Appel M, Norton E, Paoletti E (1992)Virol 187:321–328.
Alkhatib G, Briedis, DJ (1988)J Virol 62:2718–2727.
Alkhatib G, Briedis, DJ (1990)Virol 175:262–270.
Fooks AR, Shaddick E, Wames A, Dowsett BA, Rima BK, Steward M, Stephenson JR, Wilkinson GWG. Submitted.
Fooks AR, Stephenson JR, Wames A, Dowsett BA, Rima BK, Wilkinson GWG (1993)J Gen Virol 74:1439–1444.
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© 1995 Springer Science+Business Media Dordrecht
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Fooks, A.R. et al. (1995). Preliminary Studies With Scaleable Systems for the Production of Replication-Deficient Recombinant Adenoviruses in Mammalian Cell Lines. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_4
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DOI: https://doi.org/10.1007/978-94-011-0437-1_4
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