Abstract
Using different strategies, three mouse hybridoma lines (IgGl, IgG2a, IgM), were adapted to grow in three different serum-free media (BioWHITTAKER): UltraCULTURETM, U1traDOMATM and UltraDOMA-PFTM. According to the characteristics of clones, adaptation to UltraDOMATM and UltraDOMA-PFTM started from UltraCULTURETM and/or FBS-DMEM grown cells. Cell growth, antibody producing activity on a per cell basis and karyological analysis were regularly checked all along the adaptation procedure and over a several month period after transferring cells to serum-free conditions. Results are discussed with respect to the cell line, the cell generation number and the definition of the cell culture environment.
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© 1995 Springer Science+Business Media Dordrecht
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Kessler, N., Thomas, G., Bertrand, S., Aymard, M. (1995). Serum-Free Media: Influence of The Definition of The Cell Culture Environment on Stability and Antibody Productivity of Mouse Hybridomas. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_33
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DOI: https://doi.org/10.1007/978-94-011-0437-1_33
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4195-9
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