Abstract
Both core and NS5 of the Hepatitis C virus were expressed in a baculovirus system for the production of protein in serum free medium. Optimum conditions for growth ofSpodoptera frugiperdaSF-9 cells were determined in 250 ml Erlenmeyer flask culture. The cell growth was then scaled up to 1 litre using a round-bottomed bioreactor with a marine impeller. When a sufficient cell number was achieved the cells were infected with the baculovirus and the recombinant protein was characterised on SDS-PAGE and ELISA. Various parameters were investigated during the cell growth.
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© 1995 Springer Science+Business Media Dordrecht
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Charon, C.M. (1995). Infection of the Insect Cells. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_21
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DOI: https://doi.org/10.1007/978-94-011-0437-1_21
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4195-9
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