Abstract
For the design of production processes with mammalian cell lines not only cultivation strategies but also nutrient requirements and waste production are of particular importance. The determination of the specific uptake rates of all relevant substrates including glucose, glutamine and amino acids is necessary to optimize the feeding rates for processes such as fed batch or perfusion. The concentration of waste products such as ammonium and lactate and other inhibitors also play an important role for growth rate and cell death. Data from a wide variety of cell lines including CHO, mouse hybridomas, rat hybridomas and human lymphocytes were collected and specific uptake rates were calculated and compared.
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© 1995 Springer Science+Business Media Dordrecht
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Büntemeyer, H. et al. (1995). Comparison of Nutrient Requirements of Mammalian Cell Lines. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_19
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DOI: https://doi.org/10.1007/978-94-011-0437-1_19
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4195-9
Online ISBN: 978-94-011-0437-1
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