Abstract
The effect of solvent environment can overwhelm the propensity of an amino acid sequence for a particular secondary structure. Equivocal sequences that are predicted to be α-helical from amino acid preference but are found to be ß-strand from x-ray diffraction of their respective proteins, and equivocal sequences that are predicted to be ß-strand but found to be a-helical, are shown by circular dichroism (CD) to be α-helical in alcohol solvents and B-strand in hydrophobic solvents. Some sequences have a strong enough propensity to overcome environmental factors, but most sequences with a propensity for a particular secondary structure assume the structure dictated by the environment These results are experimental proof that environment as well as sequence is important in determining secondary structure. This implies that the microsolvent (environment) seen by a secondary structure due to nonlocal interactions of amino acids from the tertiary structure of a protein may be an important factor in determining secondary structure. Therefore environment should be considered to correctly predict the secondary structure of an amino acid sequence in proteins.
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References
Dill, K.A. (1990) Dominant Forces in Protein Folding, Biochemistry 29, 7133–7155.
Marqusee, S. and Baldwin, R.L. (1987) Helix stabilization by gly-lys salt bridges in short peptides of de novo design, Proc. Natl. Acad. Sci. USA 84, 8898.
Waterhous, D. V. and Johnson, W.C.Jr. (1994) Importance of environment in determining secondary structure in proteins, Biochemistry, 33, 2121–2128.
Zhong, L. and Johnson, W.C.Jr. (1992) Environment affects amino acid preference for secondary structure, Proc. Nad. Acad. Sci. USA 89, 4462–4465.
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© 1995 Springer Science+Business Media Dordrecht
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Johnson, W.C., Zhong, L., Waterhous, D.V. (1995). Environment as Well as Sequence Determines the Secondary Structure of Proteins. In: Merlin, J.C., Turrell, S., Huvenne, J.P. (eds) Spectroscopy of Biological Molecules. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0371-8_39
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DOI: https://doi.org/10.1007/978-94-011-0371-8_39
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