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Selecting the optimum genetic background for transgenic varieties, with examples from Brassica

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The Methodology of Plant Genetic Manipulation: Criteria for Decision Making

Part of the book series: Developments in Plant Breeding ((DIPB,volume 3))

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Summary

The performance of transgenic varieties depends not only upon the stable and correctly-regulated expression of specific transgenes but also upon the agronomic potential of the background genotype. Ideally, transgenes should be introduced into agronomically-superior cultivars and transgenic varieties will become out-classed if their agronomic properties are not continually improved. It will often prove desirable to use conventional breeding techniques, as opposed to new cycles of transformation, to carry out this process of varietal improvement.

Continuing advances in marker-assisted selection have made possible the selection and manipulation of an entire genetic background. This means that transgenes can be transferred to new and often ’untransformable’ varieties with relative ease. To carry out this process efficiently requires the correct choice of male and female parents, the use of appropriate marker-systems and the concentration of selection on the most appropriate generations.

Efficient, phenotypically-neutral marker-systems have revolutionised the identification and manipulation of quantitative trait loci (QTLs). The loci which modify the expression of transgenes are a form of QTL. Desirable alleles at modifier QTLs can be transferred to new varieties along with the transgenes themselves, using marker- assisted breeding.

The strategies for marker-assisted selection are becoming ever more sophisticated. A range of complementary marker systems allows the selection of desirable genotypes. In addition, the meiotic reassortment and recombination of chromosomes which produces new genotypes is becoming better understood. The most efficient plant breeding methods and the most powerful genetics will make optimal use of both markers and meiosis.

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Author notes

  1. Ulf Lagercrantz

    Present address: Department of Plant Breeding, Swedish University of Agricultural Science, Box 7003, S-750 07, Uppsala, Sweden

Authors and Affiliations

  1. Brassica and Oilseeds Research Department, John Innes Centre, Norwich, NR4 7UH, UK

    Derek Lydiate, Phil Dale, Ulf Lagercrantz, Isobel Parkin & Phil Howell

Authors
  1. Derek Lydiate
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  2. Phil Dale
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  3. Ulf Lagercrantz
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  4. Isobel Parkin
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  5. Phil Howell
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Editors and Affiliations

  1. Department of Plant Science, University College, Cork, Ireland

    Alan C. Cassells  & Peter W. Jones  & 

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© 1995 Springer Science+Business Media New York

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Lydiate, D., Dale, P., Lagercrantz, U., Parkin, I., Howell, P. (1995). Selecting the optimum genetic background for transgenic varieties, with examples from Brassica . In: Cassells, A.C., Jones, P.W. (eds) The Methodology of Plant Genetic Manipulation: Criteria for Decision Making. Developments in Plant Breeding, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0357-2_43

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  • DOI: https://doi.org/10.1007/978-94-011-0357-2_43

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4159-1

  • Online ISBN: 978-94-011-0357-2

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