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Direct and simultaneous extraction of DNA and RNA from soil

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Molecular Microbial Ecology Manual

Abstract

The direct extraction of DNA and RNA from soil is important for molecular ecological studies of the terrestrial environment. The structure and activities of natural bacterial communities can be better understood by analyses of their nucleic acids when the latter are recovered from soil samples by direct lysis instead of isolating them from bacterial cells, which have been separated from soil. One reason for this is that bacteria often are sticking strongly to soil particles and only part of them can be extracted [18, 30, 37]. On the other hand, most of the environmental bacteria, even when they are well extracted from the soil particles, fail to be cultured on laboratory media [6, 10, 44, 45]. In addition, the natural population densities of many bacteria could be rather low [22, 24].

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Author information

Authors and Affiliations

  1. Department ol Genetics, University of Bayreuth, Mansfield Road, Bayreuth, D-95440, Germany

    Sonja Selenska-Pobell

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  1. Sonja Selenska-Pobell
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Editors and Affiliations

  1. Department of Microbiology, Wageningen Agricultural University, The Netherlands

    Antoon D. L. Akkermans

  2. IPO-DLO, Wageningen, The Netherlands

    Jan Dirk Van Elsas

  3. MSU-DOE Plant Research Lab, NSF Center for Microbial Ecology and Department of Microbiology, Michigan State University, USA

    Frans J. De Bruijn

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© 1995 Springer Science+Business Media Dordrecht

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Selenska-Pobell, S. (1995). Direct and simultaneous extraction of DNA and RNA from soil. In: Akkermans, A.D.L., Van Elsas, J.D., De Bruijn, F.J. (eds) Molecular Microbial Ecology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0351-0_11

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  • DOI: https://doi.org/10.1007/978-94-011-0351-0_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4156-0

  • Online ISBN: 978-94-011-0351-0

  • eBook Packages: Springer Book Archive

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