Skip to main content

Zinnia mesophyll culture system to study xylogenesis

  • Chapter
Plant Tissue Culture Manual

Abstract

Xylem cells are formed from the procambium of the root and shoot in the primary xylem and from the vascular cambium in the secondary xylem. The main components of xylem cells are tracheary elements, which are characterized by the formation of secondary cell walls that thicken with annular, spiral, reticulate or pitted patterns. At maturity, differentiating tracheary elements lose their nuclei and cell contents, leaving behind hollow tubes that form vessels and trac-heids. In vitro, tracheary elements can be induced from the parenchymatous cells of various plant species by wounding and/or the application of phytohormones [1].

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Special cover Book
USD 54.99
Price excludes VAT (USA)
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Fukuda, H (1992) Tracheary element differentiation as a model system of cell differentiation. Int Rev Cytol 136: 289–332.

    Article  CAS  Google Scholar 

  2. Fukuda H, Komamine A (1980) Establishment of an experimental system for the tracheary element differentiation from single cells isolated from the mesophyll of Zinnia elegans. Plant Physiol 65: 57–60.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  3. Kohlenbach HW, Schmidt B (1975) Cytodifferenzierung in Form einer direkten Umwandung isolierter Mesophyll-Zellen zu Tracheiden. Z Pflanzenphysiol 75: 369–374.

    Article  Google Scholar 

  4. Fukuda, H (1994) Redifferentiation of single mesophyll cells into tracheary elements. Int J Plant Sci 155: 262–271.

    Article  CAS  Google Scholar 

  5. Sugiyama M, Komamine A (1990) Transdifferentiation of quiescent parenchymatous cells into tracheary elements. Cell Differ Dev 31: 77–87.

    Article  CAS  PubMed  Google Scholar 

  6. Fukuda H, Yoshimura T, Sato Y, Demura T (1993) In: Komamine A et al (Eds.) Molecular mechanism of xylem differentiation, pp 93–107, J Plant Res Special Issue 3, The Botanical Society of Japan, Tokyo.

    Google Scholar 

  7. Demura T, Fukuda H (1994) Novel vascular cell-specific genes whose expression is regulated temporally and spatially during vascular system development. Plant Cell 6: 967–981.

    CAS  PubMed Central  PubMed  Google Scholar 

  8. Fukuda H, Komamine A (1982) Lignin synthesis and its related enzymes as markers of tracheary-element differentiation in single cells isolated from the mesophyll of Zinnia elegans. Planta 155: 423–430.

    Article  CAS  PubMed  Google Scholar 

  9. Church DL, Galston AW (1988) Kinetics of determination in the differentiation of isolated mesophyll cells of Zinnia elegans to tracheary elements. Plant Physiol 88: 92–96.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Sugiyama M, Fukuda H, Komamine A (1986) Effects of nutrient limitation and gamma-irradiation on tracheary element differentiation and cell division in single mesophyll cells of Zinnia elegans. Plant Cell Physiol 27: 601–606.

    CAS  Google Scholar 

  11. Lin Q, Northcote DH (1990) Expression of phenylalanine ammonia-lyase gene during tracheary-element differentiation from cultured mesophyll cells of Zinnia elegans L. Planta 182: 591–598.

    Article  CAS  PubMed  Google Scholar 

  12. Siegel, SM (1953) On the biosynthesis of lignins. Physiol Plant 6: 134–139.

    Article  CAS  Google Scholar 

  13. Kuroiwa T, Suzuki T, Ogawa K, Kawano S (1981) The chloroplast nucleus: distribution, number, size, and shape, and a model for the multiplication of the chloroplast genome during chloroplast development. Plant Cell Physiol 22: 381–396.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Sugiyama, M., Fukuda, H. (1995). Zinnia mesophyll culture system to study xylogenesis. In: Lindsey, K. (eds) Plant Tissue Culture Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0303-9_5

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-0303-9_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-011-7653-8

  • Online ISBN: 978-94-011-0303-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics