Abstract
One of the most important approaches to studying embryo development is through embryo manipulation, which depends critically on the availability of an in vitro system. Three systems may be used for the manipulation — cultivation of zygotic embryos [10, 11], somatic embryos [3, 4, 19, 20, 25] and pollen embryos [8]. The advantages of using zygotic embryos rather than embryos induced from the somatic cells and pollens are their uniform pattern formation and the presence of a suspensor, which can be used as a visual indicator for the polarity of a globular proembryo. Somatic embryogenesis at the early stage is often characterised by cell proliferation that bears little resemblance to the polarized and highly regulated cell division in zygotic embryogenesis [2].
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© 1995 Springer Science+Business Media Dordrecht
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Liu, CM., Xu, ZH., Chua, NH. (1995). In vitro culture of Brassica juncea zygotic proembryo. In: Lindsey, K. (eds) Plant Tissue Culture Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0303-9_2
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DOI: https://doi.org/10.1007/978-94-011-0303-9_2
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-011-7653-8
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