Abstract
The diel changes of the biochemical composition of particulate matter was studied in Villerest reservoir (located on the Loire river, near the city of Roanne, France) during July 92. Several biomass and metabolic indicators (proteins, carbohydrates, lipids, chlorophyll a and primary production) were assessed bihourly over 2 days.
Since the P/C is largely recognized as a good integrator of the metabolic functions of the cells, we examined its distribution pattern concomitantly with aforementioned parameters. The results demonstrated enhanced P/C ratios clearly indicating that nutrients were sufficiently available for growth. In addition, this index showed a diel significant variation with levels higher in the night than in the day. Moreover, these results suggest that phytoplankton species during the night used the day-synthesized carbohydrates to insure the cell metabolic functioning.
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References
Aleya, L.,1991. Ecophysiological significance of the dielbiochem-ical changes of particulates coupled with metabolic andenvi-ronmental parameters in two trophically different lakes. Arch. Hydrobiol. 120:403–432.
Aleya, L., 1992. The seasonal succession ofphytoplankton in an eutrophic lake through the coupling of biochemicalcomposition of particulates, metabolic indicators and environmental condi-tions. Arch.Hydrobiol. 124: 69–88.
Aleya, L. &J. Devaux, 1989. Interêts et signification écophysiologiquede l’estimation de la biomasse et de l’activité photosynthétique dediverses fractions de taille phytoplanc-toniquesen milieu lacustre eutrophe. Revue Sci. Eau 2: 353–272.
Aleya, L., F. Desmolles, M. Michard, M. P. Bonnet & J. Devaux, 1993. Thedeterministic factors of the Microcystis aeruginosablooms over a biyearly survey in thehypereutrophic reservoir of Villerest(Roanne, France). Arch. Hydrobiol. (in press).
Amblard, C. & G. Bourdier, 1990 The spring bloom ofthe diatom Melosira italicasubsp. subarcticain Lake Pavin:biochemical, energetic and metabolic aspects during sedimentation. J.Plankton Res.12: 649–660.
Amenta,J. S., 1964. A rapid chemical method for quantification of lipids separated by thin layer chromatography. J. Lip. Res. 5: 270–272.
Arlova, L., 1984. Diel primary production and thevertical distribu-tion of phytoplankton in apolyhumic lake. Arch. Hydrobiol.101: 503–519.
Barlow, R. G., 1980. The biochemical composition ofphytoplankton inan upwelling region of South Africa. J. exp. mar. Biol. Ecol. 45: 83–93.
Barlow, R. G., 1982. Phytoplankton ecology in thesouthern Benguela Current. Biochemical composition. J. exp. mar. Biol.Ecol. 63: 209–228.
Bruno, S. F., R.D. Stakor, G. M. Sharma & J. T. Turner, 1983. Primary productivity and phytoplankton size fraction dominance ina temperate North Atlantic estuary. Estuaries 6: 200–211.
Cuhel, R. L. & R. S. Lean, 1987. Influence of light intensity,light quality, temperature, and daylength on uptake andassimilation of carbon dioxide and sulfate by lake plankton. Can. J.Fish. aquat. Sci.44:2118–2132.
De Laoura, I. C,J. P. Dubacq & J. C. Thomas, 1987. The effects of nitrogen deficiency on pigments and lipids of Cyanobacteria. PlantPhysiol. 83: 838–843.
Dorth, Q., J. R. Clayton, S. S. Thoresen & S. I. Ahmed, 1984.Species differencies in accumulation of nitrogen pools inphytoplankton. Mar.Biol., 81:237–250.
Fahnenstiel, G. L., L. Sicko-Goad, D. Scavia & E. F. Stoermer,1986. Importance of picoplankton in Lake Superior. Can. J. Fish. aquat. Sci. 43: 235–240.
Flynn, K. J. & P. J. Svrett, 1986. Development of the ability totake up L-lysine by the diatom Phaeodactylum tricornutum. Mar.Biol. 89:317–325.
Folch, J., M. Lees & G. H. Sloane-Stanley, 1956. A simple methodfor the isolation and purification of total lipids from animal tis-sues. J. Biol.Chem. 226: 497–509.
Ganf, G. G., S. J. L. Stone & R. L. Oliver, 1986. Use of protein to carbohydrate ratios to analyse for nutrient deficiency in phyto-plankton. Aust.J. Mar. Freshwat. Res. 37: 183–197.
Golterman, H. L., R. S. Clymo & M. A. M. Ohnstad, 1978. Methods for physical andchemical analyses of freshwaters. IBP Manual N°8 (second Edition). Blackwell Scientific Publication, Oxford, 213 pp.
Harding, L. W., B. W. Meeson & T. R. Fisher jr, 1985. Patterns photosynthetic carbon metabolism in light-limited phytoplank-ton. Mar. Biol.89: 121–133.
Joergensen, N. O. G., M. Soendergaard, H. J. Hansen, S. Bosselmann & B. Riemann, 1983. Diel variation in concentration, assimila-tionand respiration of dissolved free amino acids in relation to planktonic primaryand secondary production in two eutrophic lakes. Hydrobiologia 107: 107–122.
Kalff, J., 1972.Netplankton and nanoplankton production and biomassin a North temperate zone lake. Limnol. Oceanogr. 17: 712–720.
Lancelot, C. & S. Mathot, 1985. Biochemical fractionation of prima-ry production by phytoplankton in Belgian coastal waters during short-and long-term incubations with C-bicarbonate. I. Mixed diatompopulation. Mar. Biol. 86: 219–226.
Legendre, L. & W. D. Watt, 1971–1972. On rapidtechnic for plank-ton enumeration. Ann. Inst. Oceanogr. Paris WLVIII:173–177.
Lohman, H., 1908. Untersuchungen zur Feststellung desvollstandi-gen Gehaltes des Meeres an Plankton. -Wiss. Meeresunters, Abt. Kiel N.F, 10:132–170.
Lowry, O. H., N. J. Rosebrough, A. L. Farr & R. J. Randall, 1951. Protein measurement with the Folin phenol regent. J. Biol. Chem. 193: 265–275.
Michard, M. &L. Aleya, 1994. The mass occurrence of the cyanobacteriaMicrocystis aeruginosain the hypertrophic reser-voir of Villerest(Roanne, France): Biochemical composition of the particulate matter, metabolic activity of phytoplankton and environmentalconditions. Arch. Hydrobiol. (in press).
Moal, J., J. F. Samain, J. F. Le Coz & J. Y. Daniel, 1985.Protéines, glucides et lipides particulaires: aspects méthodologiques. Ocea-nis 11: 487–502.
Morris, I. & W. Skea, 1978. Products ofphotosynthesis in natural populationsof marine phytoplankton from the Gulf of Maine. Mar. Biol. 47: 303–312.
Munawar, M., I. F. Munawar, L. R. Culp & G. Dupuis, 1978. Rela-tiveimportance of nanoplankton in lake Superior phytoplankton biomass and communitymetabolism. J. Great Lakes Res. 4: 462– 480.
Pick, D., 1987. Carbohydrate and protein content of lake seston in relation to plankton nutrient deficiency. Can. J. Fish. aquat. Sci. 44:2097–2101.
Pinel-Alloul, B., J. Devaux, C. Amblard, G. Bourdier, O.Marvalin, N. Angeli, M. Gawler & D. Pont, 1989. Variations a court terme des compartiments planctoniques d’un lac humique du Boucher Canadien. RevueSci. Eau 2: 755–775.
Priscu,J. C, L. R. Priscu, C. Howard-Williams & W. F. Vincent, 1988. Diel patterns of photosynthate biosynthesisby phytoplank-ton in permanently ice-covered Antarctic lakes under continuous sunlight.J. Plankton. Res. 10: 333–340.
Rivkin, R. B. & M. Putt, 1987. Heterotrophy and photoheterotrophy byAntarctic microalgae: light dependent incorporation of amino-acids andglucose. J. Phycol. 23: 124–132.
Scor-Unesco, 1966. Determination of phytoplankton pigments in sea water.SCORUNESCO, 69 pp.
Spodniewska, I., 1983. Ecological characteristics of lakes in North-Eastern Poland versus their trophic gradient. VI. The phytoplank-ton of 43 lakes.Ekol. pol. 31: 353–382.
Steemann-Nielsen, E., 1977. The carbon 14 technique formeasuring organic production by plankton algae. A report on the present knowledge. Folialimnol. scand. 17: 45.
Terry, K. L., J.Hirata & E. A. Laws, 1983. Light-limited growth of two strains of the marine diatom Phaeodactylum tricornutumBohlin:chemical composition, carbone partitioning and the diel periodicity ofphysiological processes. J. exp. mar. Biol. Ecol. 68: 209–227.
Tilzer, M. M., 1973. Diurnal periodicity in the phytoplankton assem-blage of a lightmountain lake. Limnol. Oceanogr. 18: 15–30.
Utermöhl, H., 1958. Zur Vervollkommung der quantitativePhytoplankton-Methodik. Mitt. int. Ver. Limnol. 9: 1–38.
Watson, S. &J. Kalff, 1981. Relationships between nanoplankton and lake trophic status.Can. J. Fish. aquat. Sci. 38: 960–967.
Weiler, C. S. & D. M. Karl, 1979. Diel changes in phased-dividingcultures of Ceratium furca(Dinophyceae): nucleotides triphos-phate,adenylate energy charge, cell carbon, and pattern of verti-cal migration. J.Phycol. 15: 384–391.
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Michard, M., Aleya, L., Devaux, J. (1995). Diel changes in the biochemical composition of the particulate matter coupled with several parameters in the hypereutrophic Villerest reservoir (Roanne, France). In: Balvay, G. (eds) Space Partition within Aquatic Ecosystems. Developments in Hydrobiology, vol 104. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0293-3_7
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DOI: https://doi.org/10.1007/978-94-011-0293-3_7
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