Skip to main content

Diel changes in the biochemical composition of the particulate matter coupled with several parameters in the hypereutrophic Villerest reservoir (Roanne, France)

  • Conference paper
Space Partition within Aquatic Ecosystems

Part of the book series: Developments in Hydrobiology ((DIHY,volume 104))

  • 94 Accesses

Abstract

The diel changes of the biochemical composition of particulate matter was studied in Villerest reservoir (located on the Loire river, near the city of Roanne, France) during July 92. Several biomass and metabolic indicators (proteins, carbohydrates, lipids, chlorophyll a and primary production) were assessed bihourly over 2 days.

Since the P/C is largely recognized as a good integrator of the metabolic functions of the cells, we examined its distribution pattern concomitantly with aforementioned parameters. The results demonstrated enhanced P/C ratios clearly indicating that nutrients were sufficiently available for growth. In addition, this index showed a diel significant variation with levels higher in the night than in the day. Moreover, these results suggest that phytoplankton species during the night used the day-synthesized carbohydrates to insure the cell metabolic functioning.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aleya, L.,1991. Ecophysiological significance of the dielbiochem-ical changes of particulates coupled with metabolic andenvi-ronmental parameters in two trophically different lakes. Arch. Hydrobiol. 120:403–432.

    CAS  Google Scholar 

  • Aleya, L., 1992. The seasonal succession ofphytoplankton in an eutrophic lake through the coupling of biochemicalcomposition of particulates, metabolic indicators and environmental condi-tions. Arch.Hydrobiol. 124: 69–88.

    CAS  Google Scholar 

  • Aleya, L. &J. Devaux, 1989. Interêts et signification écophysiologiquede l’estimation de la biomasse et de l’activité photosynthétique dediverses fractions de taille phytoplanc-toniquesen milieu lacustre eutrophe. Revue Sci. Eau 2: 353–272.

    Google Scholar 

  • Aleya, L., F. Desmolles, M. Michard, M. P. Bonnet & J. Devaux, 1993. Thedeterministic factors of the Microcystis aeruginosablooms over a biyearly survey in thehypereutrophic reservoir of Villerest(Roanne, France). Arch. Hydrobiol. (in press).

    Google Scholar 

  • Amblard, C. & G. Bourdier, 1990 The spring bloom ofthe diatom Melosira italicasubsp. subarcticain Lake Pavin:biochemical, energetic and metabolic aspects during sedimentation. J.Plankton Res.12: 649–660.

    Article  Google Scholar 

  • Amenta,J. S., 1964. A rapid chemical method for quantification of lipids separated by thin layer chromatography. J. Lip. Res. 5: 270–272.

    CAS  Google Scholar 

  • Arlova, L., 1984. Diel primary production and thevertical distribu-tion of phytoplankton in apolyhumic lake. Arch. Hydrobiol.101: 503–519.

    Google Scholar 

  • Barlow, R. G., 1980. The biochemical composition ofphytoplankton inan upwelling region of South Africa. J. exp. mar. Biol. Ecol. 45: 83–93.

    Article  CAS  Google Scholar 

  • Barlow, R. G., 1982. Phytoplankton ecology in thesouthern Benguela Current. Biochemical composition. J. exp. mar. Biol.Ecol. 63: 209–228.

    Article  CAS  Google Scholar 

  • Bruno, S. F., R.D. Stakor, G. M. Sharma & J. T. Turner, 1983. Primary productivity and phytoplankton size fraction dominance ina temperate North Atlantic estuary. Estuaries 6: 200–211.

    Article  CAS  Google Scholar 

  • Cuhel, R. L. & R. S. Lean, 1987. Influence of light intensity,light quality, temperature, and daylength on uptake andassimilation of carbon dioxide and sulfate by lake plankton. Can. J.Fish. aquat. Sci.44:2118–2132.

    Article  CAS  Google Scholar 

  • De Laoura, I. C,J. P. Dubacq & J. C. Thomas, 1987. The effects of nitrogen deficiency on pigments and lipids of Cyanobacteria. PlantPhysiol. 83: 838–843.

    Google Scholar 

  • Dorth, Q., J. R. Clayton, S. S. Thoresen & S. I. Ahmed, 1984.Species differencies in accumulation of nitrogen pools inphytoplankton. Mar.Biol., 81:237–250.

    Article  Google Scholar 

  • Fahnenstiel, G. L., L. Sicko-Goad, D. Scavia & E. F. Stoermer,1986. Importance of picoplankton in Lake Superior. Can. J. Fish. aquat. Sci. 43: 235–240.

    Article  Google Scholar 

  • Flynn, K. J. & P. J. Svrett, 1986. Development of the ability totake up L-lysine by the diatom Phaeodactylum tricornutum. Mar.Biol. 89:317–325.

    Google Scholar 

  • Folch, J., M. Lees & G. H. Sloane-Stanley, 1956. A simple methodfor the isolation and purification of total lipids from animal tis-sues. J. Biol.Chem. 226: 497–509.

    Google Scholar 

  • Ganf, G. G., S. J. L. Stone & R. L. Oliver, 1986. Use of protein to carbohydrate ratios to analyse for nutrient deficiency in phyto-plankton. Aust.J. Mar. Freshwat. Res. 37: 183–197.

    Article  Google Scholar 

  • Golterman, H. L., R. S. Clymo & M. A. M. Ohnstad, 1978. Methods for physical andchemical analyses of freshwaters. IBP Manual N°8 (second Edition). Blackwell Scientific Publication, Oxford, 213 pp.

    Google Scholar 

  • Harding, L. W., B. W. Meeson & T. R. Fisher jr, 1985. Patterns photosynthetic carbon metabolism in light-limited phytoplank-ton. Mar. Biol.89: 121–133.

    Article  CAS  Google Scholar 

  • Joergensen, N. O. G., M. Soendergaard, H. J. Hansen, S. Bosselmann & B. Riemann, 1983. Diel variation in concentration, assimila-tionand respiration of dissolved free amino acids in relation to planktonic primaryand secondary production in two eutrophic lakes. Hydrobiologia 107: 107–122.

    Article  CAS  Google Scholar 

  • Kalff, J., 1972.Netplankton and nanoplankton production and biomassin a North temperate zone lake. Limnol. Oceanogr. 17: 712–720.

    Article  Google Scholar 

  • Lancelot, C. & S. Mathot, 1985. Biochemical fractionation of prima-ry production by phytoplankton in Belgian coastal waters during short-and long-term incubations with C-bicarbonate. I. Mixed diatompopulation. Mar. Biol. 86: 219–226.

    Article  CAS  Google Scholar 

  • Legendre, L. & W. D. Watt, 1971–1972. On rapidtechnic for plank-ton enumeration. Ann. Inst. Oceanogr. Paris WLVIII:173–177.

    Google Scholar 

  • Lohman, H., 1908. Untersuchungen zur Feststellung desvollstandi-gen Gehaltes des Meeres an Plankton. -Wiss. Meeresunters, Abt. Kiel N.F, 10:132–170.

    Google Scholar 

  • Lowry, O. H., N. J. Rosebrough, A. L. Farr & R. J. Randall, 1951. Protein measurement with the Folin phenol regent. J. Biol. Chem. 193: 265–275.

    PubMed  CAS  Google Scholar 

  • Michard, M. &L. Aleya, 1994. The mass occurrence of the cyanobacteriaMicrocystis aeruginosain the hypertrophic reser-voir of Villerest(Roanne, France): Biochemical composition of the particulate matter, metabolic activity of phytoplankton and environmentalconditions. Arch. Hydrobiol. (in press).

    Google Scholar 

  • Moal, J., J. F. Samain, J. F. Le Coz & J. Y. Daniel, 1985.Protéines, glucides et lipides particulaires: aspects méthodologiques. Ocea-nis 11: 487–502.

    CAS  Google Scholar 

  • Morris, I. & W. Skea, 1978. Products ofphotosynthesis in natural populationsof marine phytoplankton from the Gulf of Maine. Mar. Biol. 47: 303–312.

    Article  CAS  Google Scholar 

  • Munawar, M., I. F. Munawar, L. R. Culp & G. Dupuis, 1978. Rela-tiveimportance of nanoplankton in lake Superior phytoplankton biomass and communitymetabolism. J. Great Lakes Res. 4: 462– 480.

    Article  Google Scholar 

  • Pick, D., 1987. Carbohydrate and protein content of lake seston in relation to plankton nutrient deficiency. Can. J. Fish. aquat. Sci. 44:2097–2101.

    Google Scholar 

  • Pinel-Alloul, B., J. Devaux, C. Amblard, G. Bourdier, O.Marvalin, N. Angeli, M. Gawler & D. Pont, 1989. Variations a court terme des compartiments planctoniques d’un lac humique du Boucher Canadien. RevueSci. Eau 2: 755–775.

    CAS  Google Scholar 

  • Priscu,J. C, L. R. Priscu, C. Howard-Williams & W. F. Vincent, 1988. Diel patterns of photosynthate biosynthesisby phytoplank-ton in permanently ice-covered Antarctic lakes under continuous sunlight.J. Plankton. Res. 10: 333–340.

    Article  CAS  Google Scholar 

  • Rivkin, R. B. & M. Putt, 1987. Heterotrophy and photoheterotrophy byAntarctic microalgae: light dependent incorporation of amino-acids andglucose. J. Phycol. 23: 124–132.

    Google Scholar 

  • Scor-Unesco, 1966. Determination of phytoplankton pigments in sea water.SCORUNESCO, 69 pp.

    Google Scholar 

  • Spodniewska, I., 1983. Ecological characteristics of lakes in North-Eastern Poland versus their trophic gradient. VI. The phytoplank-ton of 43 lakes.Ekol. pol. 31: 353–382.

    Google Scholar 

  • Steemann-Nielsen, E., 1977. The carbon 14 technique formeasuring organic production by plankton algae. A report on the present knowledge. Folialimnol. scand. 17: 45.

    Google Scholar 

  • Terry, K. L., J.Hirata & E. A. Laws, 1983. Light-limited growth of two strains of the marine diatom Phaeodactylum tricornutumBohlin:chemical composition, carbone partitioning and the diel periodicity ofphysiological processes. J. exp. mar. Biol. Ecol. 68: 209–227.

    Article  CAS  Google Scholar 

  • Tilzer, M. M., 1973. Diurnal periodicity in the phytoplankton assem-blage of a lightmountain lake. Limnol. Oceanogr. 18: 15–30.

    Article  Google Scholar 

  • Utermöhl, H., 1958. Zur Vervollkommung der quantitativePhytoplankton-Methodik. Mitt. int. Ver. Limnol. 9: 1–38.

    Google Scholar 

  • Watson, S. &J. Kalff, 1981. Relationships between nanoplankton and lake trophic status.Can. J. Fish. aquat. Sci. 38: 960–967.

    Article  Google Scholar 

  • Weiler, C. S. & D. M. Karl, 1979. Diel changes in phased-dividingcultures of Ceratium furca(Dinophyceae): nucleotides triphos-phate,adenylate energy charge, cell carbon, and pattern of verti-cal migration. J.Phycol. 15: 384–391.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Gérard Balvay

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer Science+Business Media Dordrecht

About this paper

Cite this paper

Michard, M., Aleya, L., Devaux, J. (1995). Diel changes in the biochemical composition of the particulate matter coupled with several parameters in the hypereutrophic Villerest reservoir (Roanne, France). In: Balvay, G. (eds) Space Partition within Aquatic Ecosystems. Developments in Hydrobiology, vol 104. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0293-3_7

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-0293-3_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4129-4

  • Online ISBN: 978-94-011-0293-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics