Abstract
Nitrification has been essentially studied as a chemical process and the studies of the microbial population have been slightly worked out. This work constitutes a first attempt to study the diversity of Nitrobacter strains with the aim of working out the genetic structure of natural populations. The Nitrobacter population structure was compared between freshwater and sediments of the same lake. Nitrobacter isolates were identified and characterized by DNA/DNA hybridization, restriction pattern of rRNA genes, PCR/RFLP analysis of the ribosomal intergenic spacer and plasmid patterns. It was shown that this lake population was split into 3 subpopulations: a specific freshwater subpopulation, a specific sediment subpopulation and a nonspecific one. A 60 MDa plasmid was detected in 3,3% of freshwater isolates. A 37 MDa plasmid was detected in 59% of sediments isolates. This 37 MDa plasmid was not associated with a genomic group, but associated with a localization: the sediments. These results suggest that this 37 MDa plasmid could be implicated in the adaptation to sediment environment.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Beaupied, H., 1969. Contribution à l’étude microbiologique de sols d’étangs de la Dombes. Thèse de 3ème cycle de la Faculté des Sciences de 1’Université de Lyon, 72 pp.
Belser, L. W. & E. L. Mays, 1982. Use of activity measurements to estimate the efficiency of viable nitrifier counts in soil and sediments. Appl. Envir. Microbiol. 43: 945–948.
Brenner, D. J., A. C McWorter, J. K. Leete Knutson &A. G. Steiger-walt, 1982. Escherichia vulneris: a new species of Enterobacte-riaceae associated with human wounds. J. Clin. Microbiol. 15: 1133–1140.
Casse, F., C. Boucher, J. S. Julliot, M. Michel &J. Denarie, 1979.Identification and characterization of large p asmids in Rhizobium meliloti using agarose gel electrophoresis. J. gen. Microbiol. 113: 229–242.
Macdonald, R. M. 1986, Nitrification in soil: introductory histo-ry. In J. I. Prosser (ed.), Nitrification. SGM IRL Press Oxford Washington: 17–38.
Meyers, J. A., D. S. Sanchez, L. P. Elwell &S. Falkow, 1976. Simple agarose gel electrophoresic method for the identification and characterization of plasmid deoxyribonucleic acid. J. Bact. 129:1171.
Mullis, K.B.& F. A.Faloona, 1987. Specific synthesis of DNA in vit-ro via a polymerase-catalized chain reaction. Methods Enzymol. 155: 335–350.
Navarro, E., M. P. Fernandez, F. Grimont, A. Clays-Josserand &R. Bardin. 1992a, Genomic Heterogeneity of the genus Nitrobac-ter. Int. J. Syst. Bact. 42: 554–560.
Navarro, E., P. Simonet, P. Normand &R Bardin, 1992b. Characteri-zation of natural populations of Nitrobacter spp. using PCR/RFLP analysis of theribosomal intergenic spacer. Arch. Microbiol. 157: 107–115.
Nei, M. &W. H. Li., 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. natn. Acad.Sci. USA. 76: 5269–5273.
Pickup, R. W., 1992. Detection of gene transfer in aquatic environ-ment. In E. M. H. Wellington &J. D. Van Elsas(eds) Genetic interactions among microorganisms in the natural environment. Pergamon Press, Oxford, New-York, Seoul, Tokyo: 145–164.
Prosser, J. I. &D. J. Cox, 1982. Nitrification. In R. G. Burns &J. H. Slater (eds), Experimental microbial ecology. Blackwell Scientific Publications, Oxford: 178–193.
Schmidt, E. L., J. A. E. Molina &C. Chiang, 1973. Isolation of chemoautotrophic nitrifiers from Moroccan soils. Bull. Ecol. Res.Comm. (Stockholm). 17: 166–167.
Schmidt, E. L.&L. W. Belser, 1982. Nitrifying bacteria. In: R. H. Miller &D. R. Keeney (eds), Method of soil analysis, 2nd edn. Am. Soc. Agron. Madisson: 1027–1042.
Schutt, C, 1990. Plasmids and their role in natural aquatic bacterial communities. In J. Overbeck &R. Chrost (eds), Aquatic micro-bial ecology biochemical and molecular approaches. Springer-Verlag, New-York: 160–183.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1995 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Navarro, E., Degrange, V., Bardin, R. (1995). Genetic structure of natural populations of Nitrobacter in an aquatic environment. In: Balvay, G. (eds) Space Partition within Aquatic Ecosystems. Developments in Hydrobiology, vol 104. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0293-3_3
Download citation
DOI: https://doi.org/10.1007/978-94-011-0293-3_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4129-4
Online ISBN: 978-94-011-0293-3
eBook Packages: Springer Book Archive