Abstract
Effects of two light intensities and different concentrations of dimethyl sulfoxide (DMSO) and glycerol on hydrated cyst hatching in Thamnocephalus platyurus were studied. A maximum of 65±6% hatching was recorded within seven days at 2500 lux continuous light regime. Hatching was at a minimum during the first two days, peaked between the third and fourth days, and decreased thereafter. Hatching success was a function of duration of light exposure. Eight percent of cysts hatched in the dark, while cysts exposed to 24 h light and subsequently incubated in the dark showed 27±2% hatching. Hatchability was significantly increased (23%) in 0.0375% DMSO and 0.0125% glycerol. Concentrations above 0.05% DMSO and 0.025% glycerol had no or a negative influence on hatching. Since low concentrations of DMSO were non-toxic, apolar compounds like Ca2+ ionophore can be dissolved in DMSO to study the role of Calcium in cyst hatching.
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© 1995 Springer Science+Business Media Dordrecht
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Murugan, G., Dumont, H.J. (1995). Influence of light, DMSO and glycerol on the hatchability of Thamnocephalus platyurus Packard cysts. In: Belk, D., Dumont, H.J., Maier, G. (eds) Studies on Large Branchiopod Biology and Aquaculture II. Developments in Hydrobiology, vol 103. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0291-9_16
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DOI: https://doi.org/10.1007/978-94-011-0291-9_16
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