Abstract
The effects of linoleic acid and bovine serum albumin on hybridoma cell growth and antibody production were investigated. In dish cultivation, linoleic acid on its own promoted cell growth when used at concentrations below 50 mg LP-1, but strongly inhibited growth at a concentration of 100 mg L-1 on more. However, linoleic acid bound to bovine serum albumin did not inhibit cell growth, even at a concentration as high as 100 mg L-1. Also, linoleic acid did not affect the specific antibody production rate, with or without bovine serum albumin. In order to elucidate the enhancement of antibody production by bovine serum albumin, fractions were prepared by ultrafiltration (98% molecular weight cut-offs, 50,000 and 17,000) and the effects of the fractionation on antibody production were studied in batch cultivation. The high-molecular-weight fraction (≧ 50,000) promoted antibody production whereas the low-molecular-weight fraction (≦ 17,000) inhibited it. In continuous cultivation, the high-molecular-weight fraction was also found to enhance antibody production.
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References
Bandyopadhyay GK, Imagawa W, Wallace D and Nandi S (1987) Linoleate metabolites enhance the in vitro proliferative response of mouse mammary epithelial cells to epidermal growth factor. J. Biol. Chem. 262: 2750–2756.
Chen RF (1967) Removal of fatty acids from serum albumin by charcoal treatment J. Biol. Chem. 242: 173–181.
Halliwell B (1988) Albumin — An important extracellular antioxidant?. Biochem. Pharmacol. 37: 569–571.
Johnson AR and Stocks RB (1971) Gas-liquid chromatography of lipids. In: Biochemistry and Methodology of Lipids, pp. 195-218.
Murakami H, Masui H, Sato GH, Sueoka N, Chow TP and Sueoka TK (1982) Growth of hybridoma cells in serum-free medium: Ethanolamine is an essential component. Proc. Natl. Acad. Sci. USA 79: 1158–1162.
Nilausen K (1978) Role of fatty acids in growth-promoting effect of serum albumin on hamster cells in vitro. Journal of Cellular Physiology 96: 1–14.
Omasa T, Kobayashi M, Nishikawa T, Shioya S, Suga K, Uemura S, Kitani Y, and Imamura Y (1991) Hybridoma culture in hollow-fiber system — The effects of growth factors —. In: R. Sasaki and K. Ikura (eds.) Animal cell culture and production of biologicals. (pp. 229–236) Kluwer Academic Publishers, Dordrecht.
Omasa T, Higashiyama K, Shioya S, and Suga K (1992a) Effects of lactate concentrations on hybridoma culture in lactate-controlled fed-batch operation Biotechnol. Bioeng. 39: 556–564.
Omasa T, Ishimoto M, Higashiyama K, Shioya S, and Suga K (1992b) The enhancement of specific antibody production rate in glucose-and glutamine-controlled fed-batch culture. Cytotech-nology 8: 75–84.
Rockwell GA, Sato GH, and McClure DB (1980) The growth requirements of SV40 virus transformed Balb/c-3T3 cells in serum-free monolayer culture. Journal of Cellular Physiology 103: 323–331.
Schmid G, Zilg H, Eberhard U, and Johannsen R (1991) Effect of free fatty acids and phospholipids on growth of and product formation by recombinant baby hamster kidney (rBHK) and Chinese hamster ovary (rCHO) cells in culture. J. of Biotechnology 17: 155–167.
Spector AA (1975) Fatty acid binding to plasma albumin. J. Lipid Res. 16: 165–179
Spieker-Polet H, and Polet H (1981) Requirement of a combination of a saturated and an unsaturated free fatty acid and a fatty acid carrier protein for in vitro growth of lymphocytes. J. Immunol. 126;949–954.
Sugahara T, Shirahata S, Yamada K and Murakami H (1991a) Purification of immunoglobulin production stimulating factor 11a derived from Namalwa cells. Cytotechnology 5: 255–263.
Sugahara T, Shirahata S, Akiyoshi K, Isobe T, Okuyama T and Murakami H (1991b) Immunoglobulin production stimulating factor-IIa (TPSF-IIa) is glyceraldehyde-3-phosphate dehydrogenase like protein. Cytotechnology 6: 115–120.
Yamada K, Ikeda I, Nakajima H, Shirahata S and Murakami H (1991) Stimulation of proliferation and immunoglobulin production of human-human hybridoma by various types of caseins and their protease digests. Cytotechnology 5: 279–285.
Yamane I, Murakami H, and Kato M (1978) Role of bovine serum albumin in a serum-free suspension cell culture medium (38823). Proc. Soc. Exp. Biol. Med. 149: 439–442.
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© 1994 Springer Science+Business Media Dordrecht
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Kobayashi, M., Kato, S., Omasa, T., Shioya, S., Suga, Ki. (1994). Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production. In: Buckland, B.C., Aunins, J.G., Bibila, T.A., Hu, WS., Robinson, D.K., Zhou, W. (eds) Cell Culture Engineering IV. Current Applications of Cell Culture Engineering, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0257-5_6
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DOI: https://doi.org/10.1007/978-94-011-0257-5_6
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