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Glycosylation and functional activity of anti-D secreted by two human lymphoblastoid cell lines

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Cell Culture Engineering IV

Part of the book series: Current Applications of Cell Culture Engineering ((CACC,volume 1))

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Abstract

Cell lines BTSN4 and BTSN5 were produced by the Epstein-Barr Virus (EBV) transformation of B-lymphocytes from the same human donor. Both secrete an anti-D monoclonal of the IgG1 subclass but these antibodies display vastly different effector activities. Specifically, anti-D from BTSN4 has a far greater activity in both monocyte-and lymphocyte-mediated ADCC reactions and causes a higher percentage of rosettes to be formed with monocyte-like U937 cells. This variation in functional activity is shown to coincide with changes in the structure of the sugar chains attached to the asparagine-297 site on the immunoglobulin heavy chain.

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Abbreviations

ADCC:

anti-body dependent cellular cytotoxicity

EBV:

Epstein-Barr virus

GlcNAc:

glucosamine

Gal:

Galactose

Man:

Mannose

Fuc:

Fucose

SA:

Sialic acid

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Author information

Authors and Affiliations

  1. Departments of Chemical Engineering, The University of Sydney, NSW 2006, Australia

    Diane Cant & John Barford

  2. Departments of Infectious Diseases, The University of Sydney, NSW 2006, Australia

    Colin Harbour

  3. NSW Red Cross Blood Transfusion Service, 153 Clarence St., Sydney, NSW 2000, Australia

    Anne Fletcher

  4. Macquarie University Centre for Analytical Biotechnology, Balaclava Road, North Ryde, NSW 2113, Australia

    Nicole Packer & Andrew Gooley

Authors
  1. Diane Cant
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  2. John Barford
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  3. Colin Harbour
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  4. Anne Fletcher
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  5. Nicole Packer
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  6. Andrew Gooley
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Editor information

Editors and Affiliations

  1. Merck Research Laboratories, Biochemical Process R&D, Rahway, New Jersey, USA

    Barry C. Buckland

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© 1994 Springer Science+Business Media Dordrecht

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Cant, D., Barford, J., Harbour, C., Fletcher, A., Packer, N., Gooley, A. (1994). Glycosylation and functional activity of anti-D secreted by two human lymphoblastoid cell lines. In: Buckland, B.C., Aunins, J.G., Bibila, T.A., Hu, WS., Robinson, D.K., Zhou, W. (eds) Cell Culture Engineering IV. Current Applications of Cell Culture Engineering, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0257-5_25

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  • DOI: https://doi.org/10.1007/978-94-011-0257-5_25

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4114-0

  • Online ISBN: 978-94-011-0257-5

  • eBook Packages: Springer Book Archive

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