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NMR of Paramagnetic Molecules: A Contribution to the Understanding of Enzymatic Mechanisms

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Bioinorganic Chemistry

Part of the book series: NATO ASI Series ((ASIC,volume 459))

Abstract

The presence of a metal ion with unpaired electrons is not a drawback for NMR as long as the electron nucleus correlation time is short enough. Actually, the hyperfine coupling allows the investigaton of metalloproteins as large as 50,000 MW which otherwise are difficult to investigate. We here report the achievements obtained in the case of Cu2Co2Superoxide dismutase (MW 32,000), peroxidases (MW 34,000–46,000), cytochrome P450 (MW 45,000). The systems with inhibitors or pseudosubstrates are also discussed.

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References

  1. NMR of Paramagnetic Molecules, Academic Press, New York, 1973

    Google Scholar 

  2. Dwek, R.A. Nuclear Magnetic Resonance in Biochemistry: Applications to Enzyme Systems, Oxford University Press, London, 1973

    Google Scholar 

  3. Banci, L., Bertini, I., Luchinat, C. & Piccioli, M. in NMR and biomolecular structure (Bertini, L., Molinari, H. & Niccolai, N. eds.) pp. 31–60, VCH, 1991

    Google Scholar 

  4. Bertini, I. & Luchinat, C. NMR of paramagnetic molecules in biological systems, Benjamin/Cummings, Menlo Park, CA,1986

    Google Scholar 

  5. Noggle, J.H. & Schirmer, R.E. The nuclear Overhauser effect, Academic Press, New York, 1971

    Google Scholar 

  6. Neuhaus, D. & Williamson, M. The nuclear Overhauser effect in structural and conformational analysis, VCH, New York, 1989

    Google Scholar 

  7. Banci, L., Bertini, L., Felli, I., Kastrau, D., Luchinat, C., Piccioli, M.& Pierattelli, R. (submitted)

    Google Scholar 

  8. Tainer, J.A., Getzoff, E.D., Beem, K.M., Richardson, J.S. & Richardson, D.C. J. Mol. Biol. 1982, 160, 181.

    Article  CAS  Google Scholar 

  9. Parge, H.E., Hallewell, R.A. & Tainer, J. () Proc. Natl. Acad. Sci. USA 1992, 89, 6109.

    Article  CAS  Google Scholar 

  10. Owens, C., Drago, R.S., Bertini, I., Luchinat, C. & Banci, L., J. Am. Chem. Soc. 1986,108,3298.

    Article  CAS  Google Scholar 

  11. Bertini, I., Luchinat, C., Owens, C. & Drago, R.S., J. Am. Chem. Soc. 1987,109, 5208.

    Article  CAS  Google Scholar 

  12. Banci, L., Bertini, I. & Luchinat, C. Nuclear and electron relaxation. The magnetic nucleus-unpaired electron coupling in solution, VCH, Weinheim,1991.

    Google Scholar 

  13. Bertini, I., Lanini, G., Luchinat, C., Messori, L., Monnanni, R. & Scozzafava, A., J. Am. Chem. Soc. 1985, 107, 4391.

    Article  CAS  Google Scholar 

  14. Banci, L., Bertini, I., Luchinat, C., Piccioli, M., Scozzafava, A. & Turano, P., Inorg. Chem. 1989,28,4650.

    Article  CAS  Google Scholar 

  15. Banci, L., Bertini, I., Luchinat, C., Piccioli, M. & Scozzafava, A. Gazz. Chim. Ital, 1993, 123, 95.

    CAS  Google Scholar 

  16. Blackburn, N.J., Hasnain, S.S., Binsted, N., Diakun, G.P., Garner, C.D. & Knowles, P.F., Biochem. J., 1984, 219, 985.

    CAS  Google Scholar 

  17. Fee, J.A. & Gaber, B.P. (1972) J. Biol. Chem.,1912, 247, 60.

    Google Scholar 

  18. Pantoliano, M.W., Valentine, J.S. & Nafie, L.A., J. Am. Chem. Soc.,1982, 104, 6310.

    Article  CAS  Google Scholar 

  19. Rotilio, G., Calabrese, L., Bossa, F., Barra, D., Finazzi Agro’, A. & Mondovi, B. Biochemistry ,1972, 77, 2182.

    Article  Google Scholar 

  20. Liebermann, R.A., Sands, R.H. & Fee, J.A., J. Biol. Chem., 1982, 257, 336.

    Google Scholar 

  21. Van Camp, H.L., Sands, R.H. & Fee, J.A., Biochim. Biophys. Acta, 1982, 704, 75.

    Article  Google Scholar 

  22. Huettermann, J., Kappl, R., Banci, I., & Bertini, L, Biochim. Biophys. Acta 1988,956, 173.

    Article  CAS  Google Scholar 

  23. Fee, J.A., Peisach, J. & Mims, W.B., J. Biol. Chem. 1981,256, 1910.

    CAS  Google Scholar 

  24. Blackburn, N.J., Strange, R.W., McFadden, L.M. & Hasnain, S.S., J. Am. Chem. Soc. 1987, 109, 7162.

    Article  CAS  Google Scholar 

  25. Banci, L., Bencini, A., Bertini, I., Luchinat, C. & Viezzoli, M.S., Gazz. Chim. Ital. 1990, 120, 179.

    CAS  Google Scholar 

  26. Ming, L.J., Banci, L., Luchinat, C, Bertini, I. & Valentine, J.S., Inorg. Chem. 1988, 27, 728.

    Article  CAS  Google Scholar 

  27. Banci, L., Bertini, I., Luchinat, C., Monnanni, R. & Scozzafava, A., Inorg. Chem.1988, 27, 107.

    Article  CAS  Google Scholar 

  28. Banci, L., Bertini, I., Luchinat, C., Scozzafava, A. & Turano, P., Inorg. Chem.1989, 28, 2377.

    Article  CAS  Google Scholar 

  29. Banci, L., Bertini, I., Luchinat, C. & Scozzafava, A., J. Biol. Chem. 1989, 264, 9742.

    CAS  Google Scholar 

  30. Paci, M., Desideri, A. & Rotilio, G., J. Biol. Chem. 1988, 263, 162.

    CAS  Google Scholar 

  31. Banci, L., Bencini, A., Bertini, I., Luchinat, C. & Piccioli, M., Inorg. Chem. 1990, 29, 4867.

    Article  CAS  Google Scholar 

  32. Bertini, I., Hiromi, K., Hirose, J., Sola, M. & Viezzoli, M.S., Inorg. Chem. 1993, 32, 1106.

    Article  CAS  Google Scholar 

  33. Dunford, H.B. in Peroxidases in Chemistry and Biology (Everse, J., Everse, K.E. & Grisham, M.B. eds.) pp. 51–84, CRC Press, Boca Raton, FL, 1991.

    Google Scholar 

  34. Bosshard, H.R., Anni, H. & Yonetani, T., in Peroxidases in Chemistry and Biology (Everse, J., Everse, K.E. & Grisham, M.B. eds.) pp. 51–84, CRC Press, Boca Raton, FL, 1991.

    Google Scholar 

  35. Poulos, T.L. & Kraut, J., J. Biol. Chem. ,1980, 255, 8199.

    CAS  Google Scholar 

  36. Finzel, B.C., Poulos, T.L. & Kraut, J., J. Biol Chem. 1984, 259, 13027.

    CAS  Google Scholar 

  37. de Ropp, J.S, Yu, L.P. & La Mar G.N., J. Biomol. NMR, 1991, 1, 175.,

    Article  Google Scholar 

  38. de Ropp, J.S, La Mar, G.N., Smith, K.M. & Langry, K.C., J. Am. Chem. Soc. 1984, 106, 4438.

    Article  Google Scholar 

  39. Thanabal, V., de Ropp, J.S & La Mar, G.N., J. Am. Chem. Soc. 1987, 109, 7516.

    Article  CAS  Google Scholar 

  40. Thanabal, V., de Ropp, J.S & La Mar, G.N., J. Am. Chem. Soc. 1986, 108, 4244.

    Article  CAS  Google Scholar 

  41. Thanabal, V., de Ropp, J.S & La Mar, G.N., J. Am. Chem. Soc. 1987, 109, 265.

    Article  CAS  Google Scholar 

  42. Thanabal, V., de Ropp, J.S & La Mar, G.N., J. Am. Chem. Soc. 1988, 110, 3027.

    Article  CAS  Google Scholar 

  43. Satterlee, J.D., Erman, J.E., La Mar, G.N., Smith, K.M. & Langry, K.C., Biochim. Biophys. Acta 1983, 743, 246.

    Article  CAS  Google Scholar 

  44. Satterlee, J.D., Erman, J.E. & de Ropp, J.S., J. Biol. Chem. 1987, 262, 11578.

    CAS  Google Scholar 

  45. Satterlee, J.D., Erman, J.E., La Mar, G.N., Smith, K.M. & Langry, K.C., J. Am. Chem. Soc. 1983, 105,2099.

    Article  CAS  Google Scholar 

  46. Satterlee, J.D., Erman, J.E., Mauro, J.M. & Kraut, J., Biochemistry 1990, 29, 8797.

    Article  CAS  Google Scholar 

  47. Banci, L., Bertini, I., Turano, P., Ferrer, J.C. & Mauk, A.G.,Inorg. Chem. 1991, 30, 4510.

    Article  CAS  Google Scholar 

  48. Banci, L., Bertini, I., Kuan, I-C., Tien, M., Turano, P. & Vila, A.J., Biochemistry 1993, 32, 13483.

    Article  CAS  Google Scholar 

  49. Wang, J., Mauro, J.M., Edwards, S.L., Oatley, S.J., Fishel, L.A., Ashford, V.A., Xuong, N. & Kraut, J., Biochemistry 1990, 29, 7160.

    Article  CAS  Google Scholar 

  50. Edwards, S.L., Raag, R., Wariishi, H., Gold, M.H. & Poulos, T.L., Proc. Natl. Acad. Sci. USA ,1993, 90, 750.

    Article  CAS  Google Scholar 

  51. Piontek, K., Glumoff, T. & Winterhalter, K., FEBS Lett. 1993, 315, 119.

    Article  CAS  Google Scholar 

  52. Poulos, T.L., Edwards, S.L., Wariishi, H. & Gold, M.H., J. Biol. Chem. 1993, 268, 4429.

    CAS  Google Scholar 

  53. Bertini, I., Turano, P. & Vila, A.J., Chem. Rev. 1993, 93, 2833.

    Article  CAS  Google Scholar 

  54. Banci, L., Bertini, L, Turano, P., Tien, M. & Kirk, T.K., Proc. Natl. Acad. Sci. USA 1991, 88, 6956.

    Article  CAS  Google Scholar 

  55. Ferrer, J.C., Turano, P., Banci, L., Bertini, I., Mauk, A.G. & Smith, K.M., Biochemistry 1994(In Press)

    Google Scholar 

  56. Banci, L., Bertini, I., Bini, T., Tien, M. & Turano, P., Biochemistry 1993, 32, 5825.

    Article  CAS  Google Scholar 

  57. Weiner, L.M., CRC Crit. Rev. Biochem. 1986, 20, 139.

    Article  CAS  Google Scholar 

  58. Poulos, T.L., Finzel, B.C. & Howard, A.J., J. Mol. Biol. 1987, 195, 687.

    Article  CAS  Google Scholar 

  59. Poulos, T.L., Finzel, B.C. & Howard, A.J., Biochemistry 1986, 25, 5314.

    Article  CAS  Google Scholar 

  60. Poulos, T.L., Finzel, B.C., Gunsalus, I.C., Wagner, G. & Kraut, J., J. Biol. Chem. 1985, 260, 16122.

    CAS  Google Scholar 

  61. Collman, J.P., Sorrell, Th.N. & Hoffmann, B.M., J. Am. Chem. Soc. 1975, 97, 913.

    Article  CAS  Google Scholar 

  62. Tang, S.C., Koch, S., Papaefthyomyou, G.C., Foner, S., Frankel, R.B., Ibers, J.A. & Holm, R.H., J. Am. Chem. Soc. 1976, 98, 2414.

    Article  CAS  Google Scholar 

  63. Collman, J.P., Sorrell, Th.N., Hodgson, K.O., Kulshrestha, A.K. & Strouse, C.E., J. Am. Chem. Soc. 1971, 99, 5180.

    Article  Google Scholar 

  64. Griffin, B.W. & Peterson, J.A., J. Biol. Chem. 1975, 250, 6445.

    CAS  Google Scholar 

  65. Philson, S.B., Debrunner, P.G., Schmidt, P.G. & Gunsalus, I.C., J. Biol. Chem. 1979, 254, 10173.

    CAS  Google Scholar 

  66. Lukat, G.S. & Goff, H.M., Biochim. Biophys. Acta 1990, 1037, 351.

    Article  CAS  Google Scholar 

  67. Keller, R.M., Wóthrich, K.J. & Debrunner, P.G., Proc. Natl. Acad. Sci. USA 1972, 69, 2073.

    Article  CAS  Google Scholar 

  68. Sato, R. & Omura, T., Cytochrome P450, Academic Press, New York, 1978.

    Google Scholar 

  69. Banci, L., Bertini, I., Eltis, L.D. & Pierattelli, R., Biophys. J. 1993, 65, 806.

    Article  CAS  Google Scholar 

  70. Banci, L., Bertini, I., Marconi, S., Pierattelli, R. & Sligar, S.G. (1994) J. Am. Chem. Soc. 1994 (In Press).

    Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Florence, Via Gino Capponi 7, 50121, Florence, Italy

    Ivano Bertini & Maria Silvia Viezzoli

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  1. Ivano Bertini
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  2. Maria Silvia Viezzoli
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Editors and Affiliations

  1. Department of General and Inorganic Chemistry, Aristotle University of Thessaloniki, Thessaloniki, 54006, Greece

    Dimitris P. Kessissoglou

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Bertini, I., Viezzoli, M.S. (1995). NMR of Paramagnetic Molecules: A Contribution to the Understanding of Enzymatic Mechanisms. In: Kessissoglou, D.P. (eds) Bioinorganic Chemistry. NATO ASI Series, vol 459. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0255-1_9

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  • DOI: https://doi.org/10.1007/978-94-011-0255-1_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4113-3

  • Online ISBN: 978-94-011-0255-1

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