Abstract
The relative importance of breakdown on the accumulation of indole alkaloids has been determined in suspension cultures of Tabernaemontana divaricata and Catharanthus roseus by the feeding of stable isotope labelled alkaloids. In all cultures a considerable amount of the alkaloid biosynthesized was broken down. The breakdown was found to be dependent on the culture period and the half-life was in the order of several days. The breakdown could not explain the difference between producing and non-producing cultures. Further it was determined that in both cultures the breakdown was due to both biotic and abiotic factors.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Auriola S, Naaranlahti T & Lapinjoki SP (1991) Synthesis of [methyl-s2H]-labelled ajmalicine, yohimbine, tabersonine and catharanthine. J. Labelled Compd. Radiopharm. 29: 117–121
Becker H (1987) Regulation of secondary metabolism in plant cell cultures. Plant Tiss. Cell Cult. 199–212
Dagnino D, Schripsema J, Peltenburg A, Verpoorte R & Teunis K (1991) Capillary gas chromatographic analysis of indole alkaloids: investigation of the indole alkaloids present in Tabernae-montana divaricata cell suspension culture. J. Nat. Prod. 54: 1558–1563
Dagnino D, Schripsema J & Verpoorte R (1993a) Alkaloid metabolism in Tabernaemontana divaricata cell suspension cultures. Phytochemistry 32: 325–329
Dagnino D, Schripsema J & Verpoorte R (1993b) Comparison of terpenoid indole alkaloid production and degradation in two cell lines of Tabernaemontana divaricata. Plant Cell Rep. 13: 95–98
Dos Santos RI, Schripsema J & Verpoorte R (1994) Ajmalicine metabolism in Catharanthus roseus suspension cultures. Phytochemistry, 35: 677–681
Linsmaier EM & Skoog F (1965) Organic growth & factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100–127
Murashige T & Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Robinson T (1974) Metabolism and function of alkaloids in plants. Science 184: 430–435
Schripsema J, Meijer AH, Van Iren F, Ten Hoopen HJG & Verpoorte R (1990) Dissimilation curves as a simple method for the characterization of growth of plant cell suspension cultures. Plant Cell Tiss. Org. Cult. 22: 55–64
Schripsema J & Verpoorte R (1992) Search for factors related to the indole alkaloid production in cell suspension cultures of Tabernaemontana divaricata. Planta Med. 58: 245–249
Schripsema J, Peltenburg-Looman A, Erkelens C & Verpoorte R (1992) Nitrogen metabolism in cultures of Tabernaemontana divaricata. Phytochemistry 30: 3951–3954
Smith E (1984) Analysis of Cinchona alkaloids by high-performance liquid chromatography. J. Chromatogr. 299: 233–244
Wink M (1987) Physiology of the accumulation of secondary metabolites with special reference to alkaloids. In: Constable F & Vasil IK (Eds) Cell Culture and Somatic Cell Genetics of Plants. Vol 4: Cell Culture in Phytochemistry (pp 17–42). Academic Press, San Diego
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Schripsema, J., Dagnino, D., Dos Santos, R.I., Verpoorte, R. (1994). Breakdown of indole alkaloids in suspension cultures of Tabernaemontana divaricata and Catharanthus roseus . In: Schripsema, J., Verpoorte, R. (eds) Primary and Secondary Metabolism of Plants and Cell Cultures III. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0237-7_26
Download citation
DOI: https://doi.org/10.1007/978-94-011-0237-7_26
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4106-5
Online ISBN: 978-94-011-0237-7
eBook Packages: Springer Book Archive