Abstract
A micro flow-system was developed which consists of a chip with an integrated sensor array and a photo-patterned seal. The flow channel was produced using hybrid technologies which allow the forming of flow chambers with less than 1 µl internal volume. The used integrated sensors for measuring glucose and lactate concentrations were produced using thin film technology and were evaluated in whole blood. The underlying measuring principle is the electrochemical oxidation on a modified platinum working electrode of H2O2 which is produced by the enzymes glucose oxidase and lactate oxidase. These enzymes were immobilised in a pHEMA membrane which can be photo patterned and additionally covered by a spacer and a catalase containing cover layer. These biosensors were tested in undiluted blood with a linear range up to 40 mmol/1 for glucose and 25 mmol/1 lactate, a 95% rise time of 20 sec and an operational life time in undiluted serum of two weeks at ambient temperature. The influence of interferents and flow conditions are negligible. Such a micro flow-system was used as a laboratory on chip for decentralised acute analysis and as micro flow analysing system for ex-vivo monitoring during diabetological tests.
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© 1995 Springer Science+Business Media Dordrecht
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Urban, G., Jobst, G., Svasek, P., Varahram, M., Moser, I., Aschauer, E. (1995). Development of a Micro Flow-System with Integrated Biosensor Array. In: Van den Berg, A., Bergveld, P. (eds) Micro Total Analysis Systems. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0161-5_32
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DOI: https://doi.org/10.1007/978-94-011-0161-5_32
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4072-3
Online ISBN: 978-94-011-0161-5
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