Abstract
Monoclonal antibodies were obtained against all part of the body of the planarian Dugesia japonica japonica except the nerve cord, though parts of the enteric canal were also non reactive. The effect of a variety of fixatives (acetone, Bouin, formalin) used on planarian tissues prior to screening with antibodies, was assessed.
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References
Fujita, S., S. L. Zipursky, S. Benzer, A. Ferrus & S. L. Shotwell, 1982. Monoclonal antibodies against the Drosophila nervous system. Proc. natn. Acad. Sci. USA 79: 7929–7933.
Reuter, M., 1988. Development and organization of nervous systems visualized by immunocytochemistry in three flatworm species. Fortschr. Zool. 36: 181–184.
Romero, R., J. Fibla, D. Bueno, L. Sumoy, M. A. Soriano & J. Bagufia, 1991. Monoclonal antibodies as markers of specific cell types and regional antigens in the freshwater planarian Dugesia (G.) tigrina. Hydrobiologia 227 (Dev. Hydrobiol. 69): 73–79.
Shirakawa, T., A. Sakurai, T. Inoue, K. Sasaki, Y. Nishimura, S. Ishida & W. Teshirogi, 1991. Production of cell-and tissue-specific monoclonal antibodies in the freshwater planarian Phagocata vivida. Hydrobiologia 227 (Dev. Hydrobiol. 69): 81–91.
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© 1995 Springer Science+Business Media Dordrecht
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Shinozawa, T., Kawarada, H., Takezaki, K., Tanaka, H., Inoue, K. (1995). Preparation of monoclonal antibodies against planarian organs and the effect of fixatives. In: Cannon, L.R.G. (eds) Biology of Turbellaria and some Related Flatworms. Developments in Hydrobiology, vol 108. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0045-8_45
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DOI: https://doi.org/10.1007/978-94-011-0045-8_45
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