Isolation and Purification of Plant Viruses

Smith, Kenneth M.

doi:10.1007/978-94-010-9653-9_4

Isolation and Purification of Plant Viruses

Kenneth M. Smith C.B.E., D.Sc., Ph.D., F.R.S.²

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Abstract

When investigating a possibly undescribed sap-transmissible virus, it is desirable to have some information on certain elementary properties of the virus before commencing more comprehensive studies on its purification. What is wanted is some information on its stability and concentration in the plant, because attempts at isolation are likely to be much more difficult if the virus in question is extremely labile or occurs in very low concentration. The three elementary tests usually carried out are to determine: (a) the ageing, or longevity in vitro, of the virus in extracted sap; (b) the thermal inactivation point; and (c) the dilution end-point.

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Authors and Affiliations

Virus Research Unit, Agricultural Research Council, Cambridge, UK
Kenneth M. Smith C.B.E., D.Sc., Ph.D., F.R.S. (Formerly Director)

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Kenneth M. Smith C.B.E., D.Sc., Ph.D., F.R.S.
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Smith, K.M. (1977). Isolation and Purification of Plant Viruses. In: Plant Viruses. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-9653-9_4

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DOI: https://doi.org/10.1007/978-94-010-9653-9_4
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-412-14740-1
Online ISBN: 978-94-010-9653-9
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