Abstract
Rat leukocytes containing 84 ± 4% polymorphonuclear leukocytes (PMNs) were obtained by peritoneal lavage 24 h after the injection of 6 ml of 12% sodium caseinate. The cells were washed and resuspended at a concentration of 1–1.3 × 107 cells/ml in Eagle’s minimum essential medium buffered to pH 7.4 with 30mmol/l HEPES. The change in particle numbers reflecting aggregation was measured by the decrease in optical density in a Payton aggregometer (37°C, 900 rev/min stirring speed). Both formyl-methionyl-leucyl-phenylalanine (FMLP), over the range 10-5-10-8 mol/1 and zymosan activated serum or plasma, over the range 0.5–10% v/v, produced dose related aggregation responses. The reaction was diminished in the presence of 0.5% human serum albumin and potentiated in the presence of cytochalasin B (optimal concentration 250 ng ml-1). The optimal cell concentration was found to be 1.2 × 107 cells/ml and the technique was highly reproducible (coefficient of variation <8.5%). The aggregation of peripheral rat PMNs in response to FMLP was significantly reduced when compared to that of elicited peritoneal cells.
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© 1980 MTP Press Limited
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Shipley, M.E., Cunningham, F.M., Smith, M.J.H. (1980). Aggregation of rat polymorphonuclear leukocytes in vitro by chemotactic factors (Abstract). In: Willoughby, D.A., Giroud, J.P. (eds) Inflammation: Mechanisms and Treatment. Inflammation: Mechanisms and Treatment, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-9423-8_30
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DOI: https://doi.org/10.1007/978-94-010-9423-8_30
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-9425-2
Online ISBN: 978-94-010-9423-8
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