Fasting Canine Biliary Secretion and the Sphincter of Oddi

Abstract

This study correlates the fasting delivery of bile acids into the duodenum with motility of the sphincter of Oddi (SO). Dogs were prepared with a functional cholecystectomy, a duodenal cannula for direct vision cannulation of the common bile duct, and 12 bipolar electrodes implanted from stomach to terminal ileum. In one set of experiments, a double lumen tube was positioned in the duodenum with one orifice at the level of the SO and a second 10 cm distally. The bile acid pool was depleted, and during a continuous IV infusion of Na taurocholate (20 uMole/min) bile acid delivery into the duodenum was assessed over 6 hours by a marker perfusion technique. In other experiments, a 2-lumen continuously perfused manometry catheter was placed to record motility in the bile duct, and SO for a period of 6 hours. Station pullthroughs of the SO were performed in each phase of the MMC.